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Plant Genomes: Methods for Genetic and Physical Mapping

  • J. S. Beckmann
  • T. C. Osborn

Table of contents

  1. Front Matter
    Pages i-xi
  2. Kimberlee K. Kidwell, Thomas C. Osborn
    Pages 1-13
  3. Robert Bernatzky, Angela Schilling
    Pages 15-33
  4. Jeffrey D. Palmer
    Pages 35-53
  5. Donna M. Shattuck-Eidens, Russell N. Bell, Timothy Helentjaris
    Pages 55-70
  6. Raymond Van Daelen, Pim Zabel
    Pages 71-100
  7. Brian M. Hauge, Howard M. Goodman
    Pages 101-139
  8. Hans M. Albertsen, Hadi Abderrahim, Daniel Cohen, Denis Le Paslier
    Pages 141-165
  9. Jean-Marc Lalouel
    Pages 167-180
  10. Jacques S. Beckmann
    Pages 239-245
  11. Back Matter
    Pages 247-250

About this book

Introduction

JACQUES S. BECKMANN & THOMAS C. OSBORN Extraordinary progress has been made in the analyses of the genetic structures of higher eukaryotic genomes. Only ten years elapsed between the initial proposals to use molecular DNA markers for the generation of a complete linkage map of the human genome [5, 17] and the first description of a 10 centimorgan map of one of its chromosomes [22], soon to be followed by others. The availability of molecular DNA markers, henceforth called genomic markers [for a review of their properties see 1, 2, 20], represents a milestone in genetics by providing the capacity for complete genetic coverage of all genomes. It is important to remember that the nature of the DNA polymorphism or of the specific method used to uncover it can be quite different for different marker loci. The genetic variation detected can be a result of a simple point mutation, a DNA insertion/deletion event, or a change in repeat copy number at some hypervariable DNA [11] or micro satellite [21] motif. Currently, the methods of detection can involve use of restriction endonucleases, nucleic acid hybridization, or DNA sequence amplification. Each of these sources of var­ iation and methods of detection can have utility for different applications. Furthermore, new approaches for the detection of DNA polymorphism are constantly emerging. The primary concern here is that the monitored poly­ morphism defines a genetic marker 'useful' for the desired application.

Keywords

DNA Organe Organelle YAC hybridization

Editors and affiliations

  • J. S. Beckmann
    • 1
  • T. C. Osborn
    • 2
  1. 1.Department of Plant Genetics and BreedingAgricultural Research Organization, The Volcani CenterBet-DaganIsrael
  2. 2.Department of AgronomyUniversity of WisconsinMadisonUSA

Bibliographic information