Plant Molecular Biology Manual

  • Stanton B. Gelvin
  • Robbert A. Schilperoort

Table of contents

  1. Front Matter
    Pages i-xix
  2. In vitro methods of gene transfer to plant cells

    1. Roland Bilang, Andreas Klöti, Martin Schrott, Ingo Potrykus
      Pages 1-16
  3. Agrobacterium-mediated gene transfer to plant cells

    1. Csaba Koncz, Norbert Martini, László Szabados, Milan Hrouda, Andreas Bachmair, Jeff Schell
      Pages 53-74
    2. Paul J. J. Hooykaas, Teresa Mozo
      Pages 75-83
    3. Nancy L. Mathis, Maud A. W. Hinchee
      Pages 115-123
  4. Selectable and screenable markers for plant transformation

    1. Geert Angenon, Willy Dillen, Marc Van Montagu
      Pages 125-137
    2. Luis Herrera-Estrella, Patricia León, Olof Olsson, H. Teemu Teeri
      Pages 139-170
    3. Yves Dessaux, Annik Petit
      Pages 171-182
  5. Nucleic acid extraction from plant tissue

    1. Scott O. Rogers, Arnold J. Bendich
      Pages 183-190
    2. Gerald E. Hall Jr., Steven Spiker
      Pages 191-202
    3. C. A. Price, Noureddine Hadjeb, Lee Newman, Ellen M. Reardon
      Pages 215-229
    4. Katharina Pawlowski, Reinhard Kunze, Sacco De Vries, Ton Bisseling
      Pages 231-243
  6. Transcription and translation systems

  7. Blotting and gene detection systems

    1. Johan Memelink, Kathleen M. M. Swords, L. Andrew Staehelin, J. Harry C. Hoge
      Pages 273-295
    2. Wolfgang Werr, Bärbel Überlacker, Bettina Klinge
      Pages 297-308
    3. Susan J. Karcher
      Pages 309-333
  8. In situ hybridization and immunodetection

    1. Nicholas B. Duck
      Pages 335-347
    2. S. Hinnisdaels, I. Farbos, J. Del-Favero, J. Veuskens, M. Jacobs, A. Mouras
      Pages 349-361
  9. Cloning and detection of DNA sequences from large DNA molecules

    1. Marjory A. Snead, Patricia L. Kretz, Jay M. Short
      Pages 363-381
    2. Antoni Rafalski, Scott Tingey, John G. K. Williams
      Pages 423-429
  10. Protein-nucleic acid interaction analyses

    1. Koji Mikami, Hisabumi Takase, Masaki Iwabuchi
      Pages 431-444
    2. Susan J. Martino-Catt, Steve A. Kay
      Pages 445-457
    3. Anna-Lisa Paul, Robert J. Ferl
      Pages 459-478
  11. Subcellular targeting of proteins

    1. Barry D. Bruce, Sharyn Perry, John Froehlich, Kenneth Keegstra
      Pages 497-511
    2. Marc A. Boutry, Didier Thomas, François Chaumont
      Pages 513-519
    3. James E. Dombrowski, Luis Gomez, Maarten J. Chrispeels, Natasha V. Raikhel
      Pages 521-549
  12. Gene tagging using transposons

    1. Wolf-Ekkehard LÖnnig, Peter Huijser
      Pages 567-581
    2. Erik A. Van Der Biezen, Mark J. J. Van Haaren, Bert Overduin, H. John J. Nijkamp, Jacques Hille
      Pages 583-598
  13. Back Matter
    Pages 599-616

About this book


Five years ago, the first edition of the Plant Molecular Biology Manual appeared. At that time, the editors felt that the field of plant molecular biology had matured to a point that the publication of a series of protocols in plant molecular biology was warranted. During the past five years, the field of plant molecular biology has expanded rapidly. This expansion is, among other things, reflected by the presence of several journals in the plant sciences, as well as by the increasing amount of plant sciences articles that are published in the more general journals. In 1991 approximately 3000 people attended the Third International Congress of Plant Molecular Biology in Tucson, Arizona, where more than 2000 posters were presented. It is also remarkable to see that nowadays botanical and physiological meetings pay a considerable amount of attention to plant molecular biology. Since the first edition of this manual appeared, we have published, yearly, a series of supplements to the original volume. These supplements covered new subjects and described new methods that had been developed. With time, however, the editors realized that the original manual plus supplements had become cumbersome to use, and we decided to publish a reorganized version of the manual.


biology molecular biology

Editors and affiliations

  • Stanton B. Gelvin
    • 1
  • Robbert A. Schilperoort
    • 2
  1. 1.Purdue UniversityWest LafayetteUSA
  2. 2.Leiden State UniversityLeidenThe Netherlands

Bibliographic information