Plant Protoplasts

A Biotechnological Tool for Plant Improvement

  • Teresa Bengochea
  • John H. Dodds

Table of contents

  1. Front Matter
    Pages i-vi
  2. Teresa Bengochea, John H. Dodds
    Pages 1-2
  3. Teresa Bengochea, John H. Dodds
    Pages 3-27
  4. Teresa Bengochea, John H. Dodds
    Pages 28-43
  5. Teresa Bengochea, John H. Dodds
    Pages 44-58
  6. Teresa Bengochea, John H. Dodds
    Pages 59-66
  7. Teresa Bengochea, John H. Dodds
    Pages 67-76
  8. Teresa Bengochea, John H. Dodds
    Pages 77-86
  9. Back Matter
    Pages 87-90

About this book


Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be­ lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.


Embryo algae bacteria biology chloroplast genetic engineering growth plants plasma membrane

Authors and affiliations

  • Teresa Bengochea
    • 1
  • John H. Dodds
    • 1
  1. 1.International Potato CenterLimaPeru

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 1986
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-94-010-8317-1
  • Online ISBN 978-94-009-4095-6
  • Buy this book on publisher's site