Molecular Microbial Ecology Manual

  • Antoon D. L. Akkermans
  • Jan Dirk Van Elsas
  • Frans J. De Bruijn

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Isolation of microbial nucleic acids

    1. Ross N. Nazar, E. Jane Robb, Tatiana Volossiouk
      Pages 1-8
    2. Hugo Ramírez-Saad, Wilma M. Akkermans, Antoon D.L. Akkermans
      Pages 9-20
  3. Detection of microbial nucleic acid sequences

  4. Identification and classification of microbes using DNA and RNA sequences

    1. Kwong-Kwok Wong, Rita Cheng, Jeffrey Saffer, David Ralph, John Welsh, Michael McClelland
      Pages 63-76
  5. Detection, identification and classification of microbes using other methods

  6. Detection of gene transfer in the environment

    1. Søren J. Sørensen, Niels Kroer, Erik Sørensen, Gitte Sengeløv, Tamar Barkay
      Pages 95-123
    2. Katja E. Hill, Julian R. Marchesi, John C. Fry
      Pages 125-152
    3. Martin Day, Julian Marchesi
      Pages 153-173
  7. Tracking of the speceific microbes in the environment

    1. Jim I. Prosser, Elizabeth A. S. Rattray, Kenneth Killham, L. Anne Glover
      Pages 175-191
    2. Jon R. Saunders, Roger W. Pickup, J. Alun Morgan, Craig Winstanley, Venetia A. Saunders
      Pages 193-204
    3. Kate J. Wilson
      Pages 205-229
    4. E. Smit, K. Wernars, J. D. Van Elsas
      Pages 231-245

About this book


For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul­ tures. We now realise that only a minor fraction of the microbial com­ munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro­ bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo­ sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.


ecology microbial ecology

Editors and affiliations

  • Antoon D. L. Akkermans
    • 1
  • Jan Dirk Van Elsas
    • 2
  • Frans J. De Bruijn
    • 3
  1. 1.Department of MicrobiologyWageningen Agricultural UniversityThe Netherlands
  2. 2.IPO-DLOWageningenThe Netherlands
  3. 3.MSU-DOE Plant Research LabMichigan State UniversityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media Dordrecht 1996
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-94-011-7660-6
  • Online ISBN 978-94-009-0215-2
  • Buy this book on publisher's site