Molecular Tools for Screening Biodiversity

Plants and Animals

  • Angela Karp
  • Peter G. Isaac
  • David S. Ingram

Table of contents

  1. Front Matter
    Pages i-xxiv
  2. DNA Extraction

    1. Front Matter
      Pages 1-1
    2. DNA Extraction: widely applicable methods

      1. De-Xing Zhang, Godfrey M. Hewitt
        Pages 5-9
      2. Julia Rueda, Rosario Linacero, Ana M. Vázquez
        Pages 10-14
      3. Roselyne Lumaret, Henri Michaud, Jean-Philippe Ripoll, Lamjed Toumi
        Pages 15-17
      4. Rosario Linacero, Julia Rueda, Ana M. Vázquez
        Pages 18-21
    3. DNA Extraction: methods for difficult species/tissues

      1. De-Xing Zhang, Godfrey M. Hewitt
        Pages 27-31
      2. Birgit Ziegenhagen, Florian Scholz
        Pages 32-35
    4. Preserved specimens

      1. De-Xing Zhang, Godfrey M. Hewitt
        Pages 41-45
      2. De-Xing Zhang, Godfrey M. Hewitt
        Pages 46-48
      3. Roger Hyam
        Pages 49-50
    5. DNA Extraction using anion-exchange chromatography and silica-gel based membranes

      1. Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian
        Pages 53-54
      2. Angela Karp, Peter G. Isaac, David S. Ingram
        Pages 54-59
      3. Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian
        Pages 64-66
      4. Simone Gauch, Ralf Hermann, Petra Feuser, Uwe Oelmüller, Helge Bastian
        Pages 67-70
  3. Basic Screening Methods

    1. Front Matter
      Pages 71-71
    2. Isozymes

      1. Gerhard Müller-Starck
        Pages 75-81

About this book

Introduction

Mark Chase There are many literature resources available to molecular biologists wishing to assess genetic variation, but the myriad of techniques and approaches potentially available to the plant breeder and the evolutionary biologist is truly bewildering, and most have never been evaluated side-by-side on the same sets of samples. Additionally, it is often not recognized that tools that are useful for breeders can often be adapted for use in evolutionary studies and vice versa, but this is generally the case. The borderline between population genetics and phylogenetics is vague and difficult to assess, and a combination of both types of tools is best when it is not clear with which area one is dealing. Furthermore, it is not now appropriate to use just one type of marker in any kind of study; most markers have the potential to misinform under certain conditions, so it is always wise to incorporate at least two different types of assessments into any project. This volume is designed to facilitate this sort of multiple approach and provides comparative data on most currently available methods so that researchers can more intelligently select those appropriate to their area of interest, regardless of whether it is in the realm of breeding or evolutionary biology.

Keywords

biodiversity classification evolution morphology phylogeny tissue

Editors and affiliations

  • Angela Karp
    • 1
  • Peter G. Isaac
    • 2
  • David S. Ingram
    • 3
  1. 1.IACR-Long Ashton Research Station, Department of Agricultural SciencesUniversity of BristolUK
  2. 2.Agrogene SAMoissy CramayelFrance
  3. 3.Regius Keeper of the Royal Botanic Garden EdinburghUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-94-009-0019-6
  • Copyright Information Springer Science+Business Media B.V. 1998
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-94-010-6496-5
  • Online ISBN 978-94-009-0019-6
  • About this book