Sample Preparation in Biological Mass Spectrometry

  • Alexander R. Ivanov
  • Alexander V. Lazarev

Table of contents

  1. Front Matter
    Pages i-xxix
  2. Traditional and Improved Techniques in Sample Preparation for Proteomics

    1. Front Matter
      Pages 1-1
    2. Xianyin Lai, Frank A. Witzmann
      Pages 3-17
    3. David Arnott, Peter S. Liu, Patricia Molina, Lilian Phu, Wendy N. Sandoval
      Pages 19-40
    4. Judit M. Nagy, Alexandria Lipka, Fiona Pereira, Nicky Marlin, Stuart Hassard
      Pages 41-60
    5. Emily A. Freeman, Alexander R. Ivanov
      Pages 77-90
  3. Methods for Improved Proteolytic Digestion

    1. Front Matter
      Pages 107-107
    2. Matthew J. Berberich, Jeffrey A. Kowalak, Anthony J. Makusky, Brian Martin, Detlef Vullhorst, Andres Buonanno et al.
      Pages 109-124
    3. Daniel Lopez-Ferrer, Kim K. Hixson, Mikhail E. Belov, Richard D. Smith
      Pages 125-139
  4. Methods for Tissue, Cell, and Organelle Preparation and Analysis

    1. Front Matter
      Pages 157-157
    2. Diem Tran, Mark Daniels, Ben Colson, Dikran Aivazian, Antonio Boccia, Ingrid Joseph et al.
      Pages 159-170
    3. Yoshinori Masuo, Misato Hirano, Junko Shibato, Hyung Wook Nam, Isabelle Fournier, Céline Mériaux et al.
      Pages 171-195
    4. WenKui Lan, Marc J. Horn, Fumihiko Urano, Andre Kopoyan, Sun W. Tam
      Pages 197-214
  5. 2D Gel-Based Proteomics

About this book

Introduction

The goal and philosophy of sample preparation in biological mass spectrometry is to reveal the actual multicomponent molecular structure of a biological specimen that can be assessed by mass spectrometry tools. Unfortunately, sample preparation is often looked at as a necessary but “boring” step which does not warrant any efforts beyond a paragraph or two in a “Methods” section; it is believed not to be conducive to innovation, and does not elicit the enthusiasm of funding agencies. Nevertheless, in recent years, the attention to sample preparation methods has risen considerably, both in Academia and in the life science industry. The aim of this book is to provide the researcher with important sample preparation strategies for a wide variety of analyte molecules, specimens, methods, and biological applications demanding mass spectrometric analysis as the detection end-point. In this volume we have compiled contributions from several laboratories that employ mass spectrometry for biological analysis.

With the latest inventions and the introduction of highly sophisticated mass spectrometry equipment, sample preparation has become an extremely important bottleneck of biomedical analysis. This book is structured as a compilation of contributed chapters ranging from step-by-step protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.

Keywords

Analytical biochemistry Mass spectrometry Proteomics and protein chemistry Protocols and novel techniques Sample preparation

Editors and affiliations

  • Alexander R. Ivanov
    • 1
  • Alexander V. Lazarev
    • 2
  1. 1., Department of Genetics and Complex DiseaHarvard School of Public Health, HarvardBostonUSA
  2. 2., Research and DevelopmentPressure BioSciences, Inc.South EastonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-94-007-0828-0
  • Copyright Information Springer Science+Business Media B.V. 2011
  • Publisher Name Springer, Dordrecht
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-94-007-0758-0
  • Online ISBN 978-94-007-0828-0
  • About this book