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Invertebrate Tissue Culture Methods

  • Jun Mitsuhashi

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XVI
  2. General Methods

    1. Front Matter
      Pages 1-1
    2. Jun Mitsuhashi
      Pages 3-14
    3. Jun Mitsuhashi
      Pages 15-24
    4. Jun Mitsuhashi
      Pages 25-32
    5. Jun Mitsuhashi
      Pages 33-44
  3. Methods for Setting Up Primary Cultures Specific to Animal Groups

    1. Front Matter
      Pages 45-45
    2. Jun Mitsuhashi
      Pages 47-71
    3. Jun Mitsuhashi
      Pages 73-86
    4. Jun Mitsuhashi
      Pages 87-90
    5. Jun Mitsuhashi
      Pages 91-99
    6. Jun Mitsuhashi
      Pages 101-105
    7. Jun Mitsuhashi
      Pages 107-115
    8. Jun Mitsuhashi
      Pages 117-120
    9. Jun Mitsuhashi
      Pages 121-141
    10. Jun Mitsuhashi
      Pages 143-151
    11. Jun Mitsuhashi
      Pages 153-160
    12. Jun Mitsuhashi
      Pages 161-195
    13. Jun Mitsuhashi
      Pages 197-201
    14. Jun Mitsuhashi
      Pages 203-207
    15. Jun Mitsuhashi
      Pages 209-219
    16. Jun Mitsuhashi
      Pages 221-229
    17. Jun Mitsuhashi
      Pages 231-236
  4. Organ Culture

    1. Front Matter
      Pages 237-237
    2. Jun Mitsuhashi
      Pages 239-268
    3. Jun Mitsuhashi
      Pages 269-278
    4. Jun Mitsuhashi
      Pages 279-279
    5. Jun Mitsuhashi
      Pages 281-282
    6. Jun Mitsuhashi
      Pages 283-285
    7. Jun Mitsuhashi
      Pages 287-292
    8. Jun Mitsuhashi
      Pages 293-294
    9. Jun Mitsuhashi
      Pages 295-297
    10. Jun Mitsuhashi
      Pages 299-302
    11. Jun Mitsuhashi
      Pages 303-303
  5. Related Techniques

    1. Front Matter
      Pages 305-305
    2. Jun Mitsuhashi
      Pages 307-319
    3. Jun Mitsuhashi
      Pages 321-323
    4. Jun Mitsuhashi
      Pages 325-335
    5. Jun Mitsuhashi
      Pages 337-340
    6. Jun Mitsuhashi
      Pages 341-345
    7. Jun Mitsuhashi
      Pages 347-351
    8. Jun Mitsuhashi
      Pages 353-355
    9. Jun Mitsuhashi
      Pages 357-365
    10. Jun Mitsuhashi
      Pages 367-368
    11. Jun Mitsuhashi
      Pages 369-377
    12. Jun Mitsuhashi
      Pages 379-386
    13. Jun Mitsuhashi
      Pages 387-400
  6. Back Matter
    Pages 401-446

About this book

Introduction

I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow­ ing cells from pupal ovaries of the emperor gum moth, Antheraea euca­ lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul­ tured. The vertebrate tissue culture methods gave me some ideas for pre­ paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec­ ond, I had to examine various culture media, especially growth-promot­ ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

Keywords

Annelida Chordata Echinodermata Insecta Mollusca Orthoptera Plathelminthes Porifera invertebrates tissue

Authors and affiliations

  • Jun Mitsuhashi
    • 1
  1. 1.Tokyo University of AgricultureSetagaya, TokyoJapan

Bibliographic information