Analytical Ultracentrifugation

Instrumentation, Software, and Applications

  • Susumu Uchiyama
  • Fumio Arisaka
  • Walter F. Stafford
  • Tom Laue

Table of contents

  1. Front Matter
    Pages i-ix
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Susumu Uchiyama, Fumio Arisaka
      Pages 3-14
    3. Fumio Arisaka, Susumu Uchiyama
      Pages 15-21
  3. AUC Instrumentation and Analysis

    1. Front Matter
      Pages 23-23
    2. Thomas M. Laue, J. Brett Austin
      Pages 25-37
    3. Tao G. Nelson, Glen D. Ramsay, Matthew A. Perugini
      Pages 39-61
    4. Peter J. Sherwood, Walter F. Stafford
      Pages 81-102
    5. Borries Demeler, Gary E. Gorbet
      Pages 119-143
  4. Hydrodynamic Modeling

  5. Applications of AUC: Material Science

  6. Applications of AUC: Biological Science

    1. Front Matter
      Pages 309-309
    2. Shane E. Gordon, Matthew A. Perugini
      Pages 329-353
    3. Stephen E. Harding, Gary G. Adams, Richard B. Gillis, Fahad M. Almutairi, Gordon A. Morris
      Pages 375-386
  7. Applications of AUC: Biopharmaceuticals

  8. AUC of High-Concentration Systems and Non-ideal Solutions

  9. New Applications of AUC

About this book


This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC.
This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included.
The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.


Protein–ligand interaction Inter-molecular interactions Macromolecules in solution Association–dissociation reaction Non-ideal solutions Biopharmaceutical formulations Biophysical behavior of solutes

Editors and affiliations

  • Susumu Uchiyama
    • 1
  • Fumio Arisaka
    • 2
  • Walter F. Stafford
    • 3
  • Tom Laue
    • 4
  1. 1.Graduate School of EngineeringOsaka UniversitySuitaJapan
  2. 2.Life Science Research CenterNihon UniversityFujisawaJapan
  3. 3.Department of NeurologyHarvard Medical SchoolBostonUSA
  4. 4.BITCUniversity of New HampshireDurhamUSA

Bibliographic information