Engineered Cell Manipulation for Biomedical Application

  • Misturu Akashi
  • Takami Akagi
  • Michiya Matsusaki

Part of the Nanomedicine and Nanotoxicology book series (NANOMED)

Table of contents

  1. Front Matter
    Pages i-viii
  2. Cell Manipulation for Control of Cell Function and Assembly for Tissue Engineering

    1. Front Matter
      Pages 1-1
    2. A. Kivelio, M. Ehrbar
      Pages 3-41
    3. Ryanto The, Shuichi Yamaguchi, Akira Ueno, Yoshitake Akiyama, Keisuke Morishima
      Pages 57-74
    4. H. Sakaguchi, N. J. Amoroso, W. R. Wagner
      Pages 75-94
    5. Akihiro Nishiguchi, Michiya Matsusaki, Misturu Akashi
      Pages 95-129
    6. Christopher Millan, Marcy Zenobi-Wong
      Pages 131-145
    7. Utako Yokoyama, Yoshihiro Ishikawa
      Pages 159-174
    8. Satoshi Hirakawa, Yuji Shirakata, Koji Hashimoto
      Pages 183-190
  3. Cell Manipulation for Control of Cell Function for Drug Delivery System

    1. Front Matter
      Pages 191-191
    2. Etsushi Kuroda, Cevayir Coban, Ken J. Ishii
      Pages 193-204
    3. Takami Akagi, Misturu Akashi
      Pages 205-228
    4. Saif Salih, Charles Patrick Case
      Pages 245-258
    5. Yasuo Yoshioka, Kazuma Higashisaka, Shin-ichi Tsunoda, Yasuo Tsutsumi
      Pages 259-271
  4. Etsushi Kuroda, Cevayir Coban, Ken J. Ishii
    Pages E1-E1

About this book


This book is the first to summarize new technologies for engineered cell manipulation. The contents focus on control of cellular functions by nanomaterials and control of three-dimensional cell–cell interactions. Control of cellular functions is important for cell differentiation, maturation, and activation, which generally are controlled by the addition of soluble cytokines or growth factors into cell culture dishes. Target antigen molecules can be efficiently delivered to the cytosol of the dendritic cells using the nanoparticle technique described here, and cellular functions such as dendritic cell maturation can be controlled easily and with precision. This book describes basic preparation of the nanoparticles, activation control of dendritic cells, immune function control, and in vivo application for various vaccination systems. The second type of control, that of cell–cell interaction, is important for tissue engineering in order to develop three-dimensional cellular constructs. To achieve in vitro engineering of three-dimensional human tissue constructs, cell–cell interaction must be controlled in three dimensions, but typical biological cell manipulation technique cannot accomplish this task. An engineered cell manipulation technique is necessary. In this book the authors describe the fabrication of nanofilms onto cell surfaces, development of three-dimensional cellular multilayers, and various applications of the cellular multilayers as three-dimensional human models. This important work will be highly informative for researchers and students in the fields of materials science, polymer science, biomaterials, medicinal science, nanotechnology, biotechnology, and biology.


Biodegradable Nanoparticles Hierarchical Cell Manipulation Layer-by-layer Nanofilms Layered Tissue Models Nanoparticle Vaccine

Editors and affiliations

  • Misturu Akashi
    • 1
  • Takami Akagi
    • 2
  • Michiya Matsusaki
    • 3
  1. 1.Osaka UniversityOsakaJapan
  2. 2.Osaka UniversityOsakaJapan
  3. 3.Osaka UniversityOsakaJapan

Bibliographic information