Single-Pollen Genotyping

  • Yuji Isagi
  • Yoshihisa Suyama

Part of the Ecological Research Monographs book series (ECOLOGICAL)

Table of contents

About this book


Pollination is one of the most important processes in plant reproduction. It directly influences reproductive success and fitness and the genetic structure of the plant population. Methods exist to infer the pattern and distance of pollen dispersal, but direct observation of the movements of individual pollen grains during pollination is not feasible owing to their small size. Single-pollen genotyping is a novel technique for genotyping a single pollen grain. In this book, the principles, the experimental protocol, and several applications of this method in studies of plant ecology, reproductive biology, and evolutionary genetics have been described. More specifically, the information is useful for the analysis of linkage disequilibrium, intraspecific genetic variation, chromosome mapping, and the origins of polyploidy. It is also essential for achieving sustainable and optimal crop yield and is vital to agriculture and forestry. Written by pioneer researchers, the book provides novel research approaches that are proving useful in a growing number of fields. This volume is intended to encourage new and continued applications of single-pollen genotyping among many disciplines in the future.


Pollination conservation genetics gene flow microsatellite plant reproduction

Editors and affiliations

  • Yuji Isagi
    • 1
  • Yoshihisa Suyama
    • 2
  1. 1.Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Graduate School of Agricultural ScienceTohoku UniversityOsakiJapan

Bibliographic information