Electroporation and Sonoporation in Developmental Biology

  • Harukazu Nakamura

Table of contents

  1. Front Matter
    Pages i-xxix
  2. Electroporation

    1. Emi Ohata, Yoshiko Takahashi
      Pages 37-42
    2. Jun Hatakeyama, Kenji Shimamura
      Pages 43-53
    3. Masanori Uchikawa, Tatsuya Takemoto
      Pages 55-71
    4. Takayuki Suzuki, Toshihiko Ogura
      Pages 85-96
    5. Hidekiyo Harada, Harukazu Nakamura
      Pages 97-104
    6. Tadashi Nomura, Masanori Takahashi, Noriko Osumi
      Pages 129-141
    7. Tomomi Shimogori, Masaharu Ogawa
      Pages 153-167
    8. Naofumi Uesaka, Yasufumi Hayano, Akito Yamada, Nobuhiko Yamamoto
      Pages 169-177
    9. Anna-Lila Kaushik, Sébastien J. D. Giroux, Michèle Klaine, Ali Jalil, Yann Lécluse, Isabelle Godin
      Pages 201-217
    10. Nicholas Haddad, Daniel Houle, Indra R. Gupta
      Pages 219-227

About this book

Introduction

In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.

Keywords

Expression RNA Tempo Vertebrate biology development developmental biology electroporation embryo gene gene expression gene transfer genes nucleic acids tissue

Editors and affiliations

  • Harukazu Nakamura
    • 1
  1. 1.Graduate School of Life Sciences/Institute of Development, Aging & CancerTohoku UniversityAoba-kuJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-4-431-09427-2
  • Copyright Information Springer Japan 2009
  • Publisher Name Springer, Tokyo
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-4-431-09426-5
  • Online ISBN 978-4-431-09427-2