Imaging Mass Spectrometry

Protocols for Mass Microscopy

  • Mitsutoshi¬†Setou

Table of contents

  1. Front Matter
    Pages i-x
  2. Innovation of Imaging Mass Spectrometry

    1. Front Matter
      Pages 1-1
    2. Mitsutoshi Setou
      Pages 3-7
  3. Planning the Sample Preparation

    1. Front Matter
      Pages 9-9
    2. Yuki Sugiura, Mitsutoshi Setou
      Pages 11-30
  4. Sample Preparation

    1. Front Matter
      Pages 31-31
    2. Ikuko Yao, Mitsutoshi Setou
      Pages 33-39
    3. Yuki Sugiura, Mitsutoshi Setou
      Pages 41-54
    4. Yuki Sugiura, Mitsutoshi Setou, Daisuke Horigome
      Pages 55-69
    5. Yuki Sugiura, Mitsutoshi Setou, Daisuke Horigome
      Pages 71-85
    6. Martin Schuerenberg, Soeren-Oliver Deininger
      Pages 87-91
  5. Instrumental Operation for IMS

    1. Front Matter
      Pages 93-93
  6. Analysis of Measurement Data

    1. Front Matter
      Pages 111-111
    2. Naofumi Hosokawa, Yuki Sugiura, Mitsutoshi Setou
      Pages 113-126
    3. Yuki Sugiura, Mitsutoshi Setou
      Pages 127-142
    4. Nobuhiro Zaima, Mitsutoshi Setou
      Pages 143-155
  7. Applications of MALDI-IMS

    1. Front Matter
      Pages 157-157
    2. Hyun Jeong Yang, Yuki Sugiura, Koji Ikegami, Mitsutoshi Setou
      Pages 159-168
    3. Naoko Goto-Inoue, Takao Taki, Mitsutoshi Setou
      Pages 169-177
  8. IMS Applications Provided by Manufacturers

    1. Front Matter
      Pages 179-179
    2. Tetsuo Kokaji
      Pages 181-198
    3. Soeren-Oliver Deininger
      Pages 199-208
    4. Motoji Oshikata, Yuki Sugiura, Naohiko Yokota, Mitsutoshi Setou
      Pages 221-231
  9. Comparison of SIMS and MALDI for Mass Spectrometric Imaging

    1. Front Matter
      Pages 233-233
  10. Back Matter
    Pages 259-262

About this book


Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of researchers in academia and industry interested in wider applications of IMS. Intended for beginners or those with limited experience with IMS technology, this book provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, instrumental operations, and data analysis, among others. By describing the foundations of IMS, this volume contributes to the ongoing development of the field and to progress in human health.


Mass Spectrometer Mass Spectrometry Microscope biomolecules drug drug research kinetics microscopy molecular imaging pharmacokinetics research tissue

Editors and affiliations

  • Mitsutoshi¬†Setou
    • 1
  1. 1.Molecular Imaging Frontier Research Center Department of Molecular AnatomyHamamatsu University School of MedicineHigashi-ku, HamamatsuJapan

Bibliographic information