Fluorescence In Situ Hybridization (FISH)

Application Guide

  • Thomas Liehr

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Thomas Liehr, Anja Weise
    Pages 1-14
  3. Required Equipment and Probes for FISH Procedures

    1. Front Matter
      Pages 15-15
    2. Ivan Y. Iourov
      Pages 17-25
    3. Michael Sommerauer, Ingrid Feuerbacher, Alexander Krause
      Pages 27-42
    4. Thomas Liehr
      Pages 43-47
    5. Thomas Liehr
      Pages 49-61
    6. Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, Nigel P. Carter
      Pages 63-79
    7. Nadezda Kosyakova, Thomas Liehr, Ahmed B. Hamid Al-Rikabi
      Pages 81-100
  4. FISH Procedure

    1. Front Matter
      Pages 107-107
    2. Thomas Liehr, Katharina Kreskowski, Monika Ziegler, Katja Piaszinski, Katharina Rittscher
      Pages 109-118
    3. Anja Weise, Thomas Liehr
      Pages 119-122
    4. Vladimir A. Trifonov, Nadezhda V. Vorobieva, Natalia A. Serdyukova, Willem Rens
      Pages 123-133
    5. Gábor Méhes, Tamás Csonka, Katalin Hegyi
      Pages 141-146
    6. Gordana Joksic, Ivana Joksic, Jelena Filipović, Thomas Liehr
      Pages 147-152
    7. Bin Ma, Naoko Tanese
      Pages 153-158
  5. Material Suited for FISH Applications in Humans

  6. Multicolor-FISH-Probe Sets (mFISH) and Immunostaining

    1. Front Matter
      Pages 225-225
    2. Thomas Liehr, Sven Hauke, Britta Meyer
      Pages 227-232
    3. Thomas Liehr, Nadezda Kosyakova
      Pages 233-239
    4. Thomas Liehr, Nadezda Kosyakova, Anja Weise
      Pages 241-247
    5. Thomas Liehr, Anja Weise, Nadezda Kosyakova
      Pages 249-255
    6. Thomas Liehr, Nadezda Kosyakova, Anja Weise, Ahmed B. Hamid Al-Rikabi
      Pages 257-259
    7. Anja Weise, Thomas Liehr
      Pages 261-269
    8. Thomas Liehr, Ahmed B. Hamid Al-Rikabi, Anja Weise
      Pages 271-274
    9. Sandra Louzada, Jun Komatsu, Fengtang Yang
      Pages 275-293
    10. Anja Weise, Thomas Liehr
      Pages 295-300
    11. Christine J. Ye, Guo Liu, Henry H. Q. Heng
      Pages 301-325
    12. Bin Ma, Naoko Tanese
      Pages 327-335
    13. Anna A. Pendina, Olga A. Efimova, Andrei V. Tikhonov, Olga G. Chiryaeva, Irina D. Fedorova, Alla S. Koltsova et al.
      Pages 337-346
    14. Elisabeth Klein, Thomas Liehr
      Pages 347-351
  7. Interphase FISH

    1. Front Matter
      Pages 353-353
    2. Thomas Liehr, Sven Hauke
      Pages 355-359
    3. Ivan Y. Iourov, Svetlana G. Vorsanova, Yuri B. Yurov
      Pages 361-372
    4. Galina Hovhannisyan, Rouben Aroutiounian
      Pages 373-378
    5. Galina Hovhannisyan, Tigran Harutyunyan, Thomas Liehr
      Pages 379-383
    6. Thomas Liehr, Nadezda Kosyakova
      Pages 385-392
  8. Applications of FISH in Zoology, Botany and Microbiology

    1. Front Matter
      Pages 393-393
    2. Fengtang Yang, Alexander S. Graphodatsky
      Pages 395-415

About this book


This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection.

This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.


Chromosomes Molecular cytogenetic diagnostics Multicolor FISH Immunostaining Microdissection Fluorescence microscopy Comparative genomic hybridization

Editors and affiliations

  • Thomas Liehr
    • 1
  1. 1.Institut für HumangenetikJenaGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-662-52957-7
  • Online ISBN 978-3-662-52959-1
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site