Hydrocarbon and Lipid Microbiology Protocols

Petroleum, Hydrocarbon and Lipid Analysis

  • Terry J. McGenity
  • Kenneth N. Timmis
  • Balbina Nogales

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Daniel J. Letinski, Roger C. Prince
    Pages 9-30
  3. Robert F. Swarthout, Jonas Gros, J. Samuel Arey, Robert K. Nelson, David L. Valentine, Christopher M. Reddy
    Pages 129-149
  4. Ian Colbeck
    Pages 151-159
  5. Zhengzhen Zhou, Laodong Guo, Christopher L. Osburn
    Pages 179-200
  6. Gabriela M. Vázquez-Cuevas, Kirk T. Semple
    Pages 231-246
  7. Yoann Le Digabel, Siham Beggah, Jan Roelof van der Meer
    Pages 247-257
  8. Lars Wörmer, Julius S. Lipp, Kai-Uwe Hinrichs
    Pages 289-317

About this book


This Volume presents methods for analysing and quantifying petroleum, hydrocarbons and lipids, based on their chemical and physical properties as well as their biological effects. It features protocols for extracting hydrocarbons from solid matrices, water and air, and a dedicated chapter focusing on volatile organic compounds. Several approaches for separating and detecting diverse classes of hydrocarbons and lipids are described, including: (tandem) gas chromatography (GC) coupled with mass spectrometry (MS) or flame-ionisation detection, Fourier-transform induction-coupled-resonance MS, and fluorescence-based techniques. The book details high-performance liquid chromatography MS for microbial lipids, as well as a combination of techniques for naphthenic acids. Two chapters focus on quantifying bioavailable hydrocarbon fractions by using cyclodextrin sorbents and bacterial bioreporters, respectively, while a closing chapter explains how compound-specific stable-isotope analysis can be used to measure the fate of hydrocarbons in the environment.

Hydrocarbon and Lipid Microbiology Protocols

There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.


Bacterial bioreporter assays Biodegradation Bioremediation and natural attenuation Hydrocarbon bioavailability Hydrocarbon extraction Naphthenic acids Petroleum biomarkers Ultra-high resolution mass spectrometry for heavy oils Volatile hydrocarbons

Editors and affiliations

  • Terry J. McGenity
    • 1
  • Kenneth N. Timmis
    • 2
  • Balbina Nogales
    • 3
  1. 1.School of Biological SciencesUniversity of EssexColchesterUnited Kingdom
  2. 2.Institute of MicrobiologyTechnical University BraunschweigBraunschweigGermany
  3. 3.Department of BiologyUniversity of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)Palma de MallorcaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2017
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-662-52791-7
  • Online ISBN 978-3-662-52793-1
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site