Cryopreservation of Plant Germplasm II

  • Leigh E. Towill
  • Y. P. S. Bajaj

Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 50)

Table of contents

  1. Front Matter
    Pages I-XVI
  2. Cryopreservation of Plant Germplasm

  3. Herbaceous Species

    1. Front Matter
      Pages 35-35
    2. M. Elena González-Benito, José María Iriondo
      Pages 48-56
    3. Kazumasa Hirata, Phunchindawan Monthana, Akira Sakai, Kazuhisa Miyamoto
      Pages 57-65
    4. Eva Čellárová, Katarína Kimáková, Martina Urbanová
      Pages 66-77
    5. B. Vandenbussche, M. Demeulemeester, M. De Proft
      Pages 78-95
    6. Nguyen Tien Thinh, Hiroko Takagi
      Pages 96-118
    7. Jun-Hui Wang, Chun-Nong Huang
      Pages 119-135
    8. M. A. Revilla, D. Martínez
      Pages 136-150
    9. Leigh E. Towill
      Pages 151-163
    10. Kayo Yoshimatsu, Koichiro Shimomura
      Pages 164-179
  4. Woody Species

    1. Front Matter
      Pages 197-197
    2. Z. Jekkel, J. Kiss, G. Gyulai, E. Kiss, L. E. Heszky
      Pages 199-212
    3. Dominique Dumet, Patricia Berjak
      Pages 213-219
    4. S. Dussert, N. Chabrillange, E. Engelmann, F. Anthony, N. Vasquez, S. Hamon
      Pages 220-233
    5. M. Pâques, V. Monod, M. Poissonnier, J. Dereuddre
      Pages 234-245
    6. M. A. Revilla, D. Martínez, J. M. Martínez-Zapater, R. Arroyo García
      Pages 257-268
    7. Marie-Thérèse de Boucaud, Marthe Brison, Bertrand Helliot, Valérie Hervé-Paulus
      Pages 287-311
    8. M. Elena González-Benito, Carmen Martín
      Pages 312-322
    9. Barbara M. Reed, Kim E. Hummer
      Pages 323-343
    10. P. T. Lynch
      Pages 344-353
  5. Australian Species

    1. Front Matter
      Pages 355-355
    2. D. H. Touchell, S. R. Turner, E. Bunn, K. W. Dixon
      Pages 357-372
    3. Darren Touchell, S. R. Turner, T. Senaratna, E. Bunn, K. W. Dixon
      Pages 373-390
  6. Back Matter
    Pages 391-396

About this book


Ex situ preservation of germplasm for higher plant species has been accom­ plished using either seeds or clones, but storage of these under typical condi­ tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur­ vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com­ monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry­ opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.


Cryopreservation Embryo Gefrierkonservierung Gewebekultur Keimplasma Neem Pflanzen germplasm olive plants rose tissue culture woody species

Editors and affiliations

  • Leigh E. Towill
    • 1
  • Y. P. S. Bajaj
    • 2
  1. 1.USDA-ARS National Center for Genetic Resources PreservationFort CollinsUSA
  2. 2.New DelhiIndia

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2002
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-07502-5
  • Online ISBN 978-3-662-04674-6
  • Series Print ISSN 0934-943X
  • Buy this book on publisher's site