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Differential-Display Reverse Transcription-PCR (DDRT-PCR)

  • Sergio Colonna-Romano
  • Antonella Leone
  • Bruno Maresca

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-X
  2. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 1-14
  3. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 15-33
  4. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 34-45
  5. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 46-54
  6. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 55-58
  7. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 59-63
  8. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 64-76
  9. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 77-80
  10. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 81-95
  11. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 96-101
  12. Sergio Colonna-Romano, Antonella Leone, Bruno Maresca
    Pages 102-109
  13. Back Matter
    Pages 110-124

About this book

Introduction

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Keywords

DNA Gene Molekulargenetik PCR RNA Zelldifferenzierung differentiation genes mRNA molecular genetics transcription

Authors and affiliations

  • Sergio Colonna-Romano
    • 1
  • Antonella Leone
    • 2
  • Bruno Maresca
    • 1
  1. 1.International Institute of Genetics and BiophysicsNational Research CouncilNaplesItaly
  2. 2.Research-Institute of Vegetable and Ornamental Plant BreedingNational Research CouncilPorticiItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-80454-0
  • Copyright Information Springer-Verlag Berlin Heidelberg 1998
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-63297-9
  • Online ISBN 978-3-642-80454-0
  • Buy this book on publisher's site