Animal Cell Culture Techniques

  • Martin Clynes

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XIII
  2. General Methods

    1. Front Matter
      Pages 1-1
    2. Shirley McBride, Mary Heenan, Martin Clynes
      Pages 3-12
    3. Edwin O’Kelly
      Pages 13-37
    4. Joanne Keenan, Paula Meleady, Martin Clynes
      Pages 54-66
    5. Joanne Keenan, Martin Clynes
      Pages 67-81
    6. Elizabeth Moran, Annemarie Larkin, Allan Masterson, Martin Clynes
      Pages 82-104
    7. Finbar O’Sullivan, Paula Meleady, Shirley McBride, Martin Clynes
      Pages 115-128
  3. Cell proliferation and Death

    1. Front Matter
      Pages 129-129
    2. Conor Duffy, Ailish Hynes, Leo Quinlan
      Pages 149-169
    3. S. Verhaegen, Seamus Coyle, Lisa M. Connolly, Colette O’Loughlin, Martin Clynes
      Pages 170-193
  4. In Vitro Models for Cell Differentiation

    1. Front Matter
      Pages 195-195
    2. Domenico Bosco, Paolo Meda
      Pages 197-216
    3. Shirley McBride, Martin Clynes
      Pages 231-239
    4. Philippe Poindron, Yves Lombard
      Pages 240-263
    5. Alan W. Baird, Ena S. Prosser
      Pages 298-318
    6. Valerie Urbach, Deirdre Walsh, Maria Higgins, Isabelle Leguen, Christina Doolan, John Cuffe et al.
      Pages 319-356
    7. Paula Meleady, Finbar O’ Sullivan, Shirley McBride, Martin Clynes
      Pages 357-370
    8. Gennady Ilyin, Anne Corlu, Pascal Loyer, Christiane Guguen-Guillouzo
      Pages 371-392
  5. In Vitro Models for Toxicology and Pharmacology

    1. Front Matter
      Pages 393-393
    2. Flavia Zucco, Isabella De Angelis, Annalaura Stammati
      Pages 395-422
    3. Robert O’Connor, Mary Heenan, Conor Duffy, Martin Clynes
      Pages 423-433
  6. Industrial Application of Animal Cell Culture

    1. Front Matter
      Pages 457-457
    2. Eunan McGlinchey, Donnacha O’Driscoll, Martin Clynes
      Pages 459-474
    3. Conor O’Dea, Suzanne O’Connor, Martin Clynes
      Pages 475-486
    4. Alice Redmond, Jacqueline Quinn, Susan Molloy
      Pages 521-541
  7. Genetic Manipulation and Analysis of Human and Animal Cells in Culture

    1. Front Matter
      Pages 543-543
    2. Daragh Byrne, Carmel Daly, Martin Clynes
      Pages 545-554
    3. Carmel Daly, Daragh Byrne, Martin Clynes
      Pages 555-566
    4. Toni O’Doherty, Róisín Nic Amhlaoibh, Martin Clynes
      Pages 582-599
    5. Gerard J. McGarrity
      Pages 600-612
  8. Back Matter
    Pages 613-618

About this book


Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.


Colon Monoclonal Antibodies cell cell culture cell cycle cell differentiation cells cytokine gene expression gene therapy leukemia metabolism pharmacology toxicology transcription

Editors and affiliations

  • Martin Clynes
    • 1
  1. 1.National Cell & Tissue Culture Center Bioresearch IrelandDublin City UniversityGlasnevin Dublin 9Ireland

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1998
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-63008-1
  • Online ISBN 978-3-642-80412-0
  • Buy this book on publisher's site