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Signal Transduction — Single Cell Techniques

  • Bert Van Duijn
  • Anneke Wiltink

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XXVII
  2. Handling of Cells in Single Cell Experiments

    1. Front Matter
      Pages 1-1
    2. Arie de Vos, Bert Van Duijn, Anneke Wiltink
      Pages 19-23
    3. Pettie P. Booij, Albertus H. De Boer
      Pages 31-40
  3. Ion Channel and Membrane Potential Measurements Using the Patch-Clamp Technique

    1. Front Matter
      Pages 41-41
    2. Introduction to the Measurement Technique

    3. Single Channel Measurements

    4. II-3 Whole Cell Measurements

      1. E. Etienne Verheijck
        Pages 207-213
      2. Rezso Gáspár Jr., Zoltan Varga, György Panyi, Zoltan Krasznai, Carlo Pieri, Sandor Damjanovich
        Pages 214-235
      3. Zoltan Krasznai, Adam F. Weidema, Rezsö Gáspár, György Panyi, Dirk L. Ypey
        Pages 236-245
  4. Fluorescence To Measure Intracellular Ions

    1. Front Matter
      Pages 283-283
    2. Introduction to the Measurement Technique

    3. Flow Cytometer Measurements

      1. Sandor Damjanovich, Carlo Pieri, Laszlo Bene, Attila Jenei, Rezsö Gáspár Jr.
        Pages 343-347
      2. Sandor Damjanovich, Carlo Pieri, Laszlo Bene, Attila Jenei, Rezsö Gáspár Jr.
        Pages 348-357
      3. Erika Baus, Jacques Urbain, Oberdan Leo, Fabienne Andris
        Pages 358-372
    4. Microfluorescence Measurements

    5. Confocal Microscopy

      1. Mark Fricker, Rachel Errington, Julian Wood, Monika Tlalka, Mike May, Nick White
        Pages 409-441
      2. Wim J. J. M. Scheenen
        Pages 442-457
  5. Back Matter
    Pages 459-468

About this book

Introduction

A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual. An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.

Keywords

ATP Calcium Endoplasmatisches Reticulum Ionentransport Lipid Monitor Zellkommunikation Zellphysiologie cell nucleus cell signaling ion channels microscope regulation surgery tissue

Editors and affiliations

  • Bert Van Duijn
    • 1
  • Anneke Wiltink
    • 2
  1. 1.Center for Phytotechnology RUL/TNOLeidenThe Netherlands
  2. 2.Department of Physiology, Academic Medical CentreUniversity of AmsterdamAmsterdamThe Netherlands

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-80368-0
  • Copyright Information Springer-Verlag Berlin Heidelberg 1998
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-48976-1
  • Online ISBN 978-3-642-80368-0
  • Buy this book on publisher's site