Table of contents
About this book
The technique of microinjection is used to tranfer biological materials - RNA, proteins, oligonucleotides and especially cloned genes - into cultured somatic cells or embryos. Transgenic cells or animals - mice, rabbits or insects - allow the study of a wide range of cellular processes, such as gene expression, signal transduction or the cytoskeleton. More than 30 protocols on microinjection experiments and transgenesis studies developed in renowned laboratories are described in this manual. Special topics are the generation of transgenic mice using YAC, ligand-dependent site-specific recombination in embryonic stem cells, morula aggregation to generate germline chimeras as well as the analysis of injected cells.
Vivo biochemistry cell cell culture chromosome electron microscopy gene gene expression gene transfer microscopy proteins ribozyme stem cells tissue transcription