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Microinjection and Transgenesis

Strategies and Protocols

  • Angel Cid-Arregui
  • Alejandro García-Carrancá
Book

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Strategies and Protocols for Microinjection Experiments Using Somatic Cells in Culture

    1. Front Matter
      Pages 1-1
    2. Angel Cid-Arregui, Carla Santana, Miriam Guido, Nestor Morales-Peza, Maria Victoria Juarez, Roger Wepf et al.
      Pages 3-30
    3. Wilhelm Ansorge, Rainer Saffrich
      Pages 31-46
    4. Gustavo Egea, Teresa Babia, Roser Pagan, Roser Busca, Inmaculada Ayala, Ferran Valderrama et al.
      Pages 47-79
    5. Jaime Berumen
      Pages 113-129
    6. Rainer Pepperkok, Olaf Rosorius, Jochen Scheel
      Pages 145-154
    7. Serge Roche, Sara A. Courtneidge
      Pages 171-183
  3. Strategies and Protocols for Microinjection Experiments Using Embryos

    1. Front Matter
      Pages 185-185
    2. Minoo Rassoulzadegan, Julien Sage, Valérie Grandjean
      Pages 217-227
    3. Marialuisa Lavitrano, Valentina Lulli, Barbara Maione, Sabina Sperandio, Corrado Spadafora
      Pages 229-254
    4. Pierre-Olivier Angrand, Catherine P. Woodroofe, A. Francis Stewart
      Pages 255-274
    5. Carol Murphy, Luis Martin-Parras, Ulrich Rüther
      Pages 275-284
    6. Holger Hiemisch, Thorsten Umland, Lluís Montoliu, Günther Schütz
      Pages 297-308
    7. Sylvie Forlani, Lucile Montfort, Jean-François Nicolas
      Pages 369-412
    8. Catherine E. Ovitt, Young Il Yeom, Hans R. Schöler
      Pages 427-437
    9. Sylvie Schneider-Maunoury, Piotr Topilko, Tania Seitanidou, Giovanni Levi, Paula Murphy, Patrick Charnay
      Pages 459-476
    10. Angel Cid-Arregui, Nestor Morales-Peza, Prasert Auewarakul, Maria Jose Garcia-Iglesias, Maria Victoria Juarez, Gina Diaz et al.
      Pages 477-504
    11. Miriam H. Meisler, James Galt, John S. Weber, Julie M. Jones, Daniel L. Burgess, David C. Kohrman
      Pages 505-520
    12. José Moreno, Laura C. Bonifaz, Jesús Martínez-Barnetche
      Pages 521-559
    13. Urban Besenfelder, Bernhard Aigner, Mathias Müller, Gottfried Brem
      Pages 561-586
    14. Cristiana Mollinari, Cayetano González
      Pages 587-603
  4. Back Matter
    Pages 605-619

About this book

Introduction

The technique of microinjection is used to tranfer biological materials - RNA, proteins, oligonucleotides and especially cloned genes - into cultured somatic cells or embryos. Transgenic cells or animals - mice, rabbits or insects - allow the study of a wide range of cellular processes, such as gene expression, signal transduction or the cytoskeleton. More than 30 protocols on microinjection experiments and transgenesis studies developed in renowned laboratories are described in this manual. Special topics are the generation of transgenic mice using YAC, ligand-dependent site-specific recombination in embryonic stem cells, morula aggregation to generate germline chimeras as well as the analysis of injected cells.

Keywords

Vivo biochemistry cell cell culture chromosome electron microscopy gene gene expression gene transfer microscopy proteins ribozyme stem cells tissue transcription

Editors and affiliations

  • Angel Cid-Arregui
    • 1
  • Alejandro García-Carrancá
    • 2
  1. 1.NeurobiologieUniversität HeidelbergHeidelbergGermany
  2. 2.Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoMexico CityMexico

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-80343-7
  • Copyright Information Springer-Verlag Berlin Heidelberg 1998
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-61895-9
  • Online ISBN 978-3-642-80343-7
  • Buy this book on publisher's site