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Cell Culture Techniques in Heart and Vessel Research

  • H. M. Piper

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Heart Muscle Cells

    1. Front Matter
      Pages 1-1
    2. A. C. Nag
      Pages 3-19
    3. A. Pinson
      Pages 20-35
    4. H. M. Piper, A. Volz, P. Schwartz
      Pages 36-60
    5. M. Cantin
      Pages 61-74
    6. S. L. Jacobson, R. A. Altschuld, C. M. Hohl
      Pages 75-98
    7. T. K. Borg, L. Terracio
      Pages 99-107
    8. P. A. Uusimaa, I. E. Hassinen
      Pages 108-114
  3. Endothelial Cells

    1. Front Matter
      Pages 115-115
    2. A. M. Rosenthal, A. I. Gotlieb
      Pages 117-129
    3. P. D. Bowman, M. du Bois, K. Dorovini-Zis, R. R. Shivers
      Pages 140-157
    4. H. M. Piper, R. Spahr, S. Mertens, A. Krützfeldt, H. Watanabe
      Pages 158-177
    5. V. W. M. van Hinsbergh, M. A. Scheffer, E. G. Langeler
      Pages 178-204
    6. D. M. Shasby, S. S. Shasby
      Pages 212-219
    7. R. Spahr, H. M. Piper
      Pages 220-229
    8. D. Gospodarowicz
      Pages 230-244
  4. Smooth Muscle Cells and Pericytes

    1. Front Matter
      Pages 245-245
    2. P. Fallier-Becker, J. Rupp, J. Fingerle, E. Betz
      Pages 247-270
    3. V. N. Smirnov, A. N. Orekhov
      Pages 271-289
    4. P. A. D’Amore
      Pages 299-314
    5. J. Thyberg, U. Hedin, B. A. Bottger
      Pages 315-333
    6. P. F. Davies, S. E. O’Connor
      Pages 334-342
    7. U. Janßen-Timmen, P. Salbach, R. Gronwald, A. J. R. Habenicht
      Pages 343-350
  5. Back Matter
    Pages 351-362

About this book

Introduction

In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

Keywords

Endothelzellen Kardiomyozyten Myokardiozyten Myokardzell-Kultur cardiovascular cells endothelium glatte Muskelzellen smooth muscle tissue

Editors and affiliations

  • H. M. Piper
    • 1
  1. 1.Physiologisches InstitutAbteilung für Herz- und Kreislaufphysiologie der Universität DüsseldorfDüsseldorf 1Germany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-75262-9
  • Copyright Information Springer-Verlag Berlin Heidelberg 1990
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-75264-3
  • Online ISBN 978-3-642-75262-9
  • Buy this book on publisher's site