Plant Protoplasts and Genetic Engineering I

  • Y. P. S. Bajaj

Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 8)

Table of contents

  1. Front Matter
    Pages I-XX
  2. Isolation, Culture, and Preservation of Protoplasts

    1. Front Matter
      Pages 1-1
    2. P. B. Gahan
      Pages 34-49
    3. J. J. M. Dons, C. M. Colijn-Hooymans
      Pages 50-62
    4. H. Schnabl, U. Zimmermann
      Pages 63-96
    5. Y. P. S. Bajaj
      Pages 97-106
  3. Regeneration of Plants from Protoplasts

    1. Front Matter
      Pages 107-107
    2. J. Kyozuka, K. Shimamoto, H. Ogura
      Pages 109-123
    3. M. Zuba, H. Binding
      Pages 124-146
    4. Aliza Vardi, Esra Galun
      Pages 147-159
    5. M. N. Barakat, E. C. Cocking
      Pages 160-172
    6. C. A. Newell, H. T. Luu
      Pages 173-192
    7. Kazunori Yabe, Takeshi Nishio, Kenji Takayanagi
      Pages 227-234
    8. Saburo Yasugi
      Pages 235-253
    9. Charlene J. Boyes, Kenneth C. Sink
      Pages 254-261
    10. Edward G. Kirby, Michael A. Campbell, Ricardo M. Penchel
      Pages 262-274
  4. Protoplast Fusion and Somatic Hybridization

    1. Front Matter
      Pages 275-275
    2. K. N. Kao, M. R. Michayluk
      Pages 277-288
    3. I. Negrutiu, A. Mouras, Y. Y. Gleba, V. Sidorov, S. Hinnisdaels, Y. Famelaer et al.
      Pages 304-319
    4. Maureen R. Hanson, Mary A. O’Connell, Charles Vidair
      Pages 320-335
    5. Z. Tabaeizadeh, C. Bergounioux, Y. Demarly
      Pages 336-355
    6. D. M. Gilmour, T. J. Golds, M. R. Davey
      Pages 370-388
    7. K. Toriyama, T. Kameya, K. Hinata
      Pages 389-396
    8. J. Honkanen, P. Ryöppy
      Pages 397-409
    9. M. Niizeki
      Pages 410-434
  5. Back Matter
    Pages 435-444

About this book


Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara­ tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter­ national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in­ corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.


Cybrid genetic engineering plant protoplast protoplasts regeneration

Editors and affiliations

  • Y. P. S. Bajaj
    • 1
  1. 1.New DelhiIndia

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 1989
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-73616-2
  • Online ISBN 978-3-642-73614-8
  • Series Print ISSN 0934-943X
  • Buy this book on publisher's site