Fluorescence Correlation Spectroscopy

Theory and Applications

  • Rudolf Rigler
  • Elliot S. Elson

Part of the Springer Series in Chemical Physics book series (CHEMICAL, volume 65)

Table of contents

  1. Front Matter
    Pages I-XX
  2. 1 Introduction

    1. E. Elson
      Pages 1-6
  3. FCS in the Analysis of Molecular Interactions

    1. Front Matter
      Pages 7-7
    2. Antonie J. W. G. Visser, Petra A. W. van den Berg, Mark A. Hink, Valentin N. Petushkov
      Pages 9-24
    3. Zeno Foldes-Pappn, Masataka Kinjo
      Pages 25-64
  4. FCS at the Cellular Level

    1. Front Matter
      Pages 99-99
    2. Alladin Pramanik, Rudolf Rigler
      Pages 101-131
  5. Applications in Biotechnology, Drug Screening, and Diagnostics

  6. Environmental Analysis and Monitoring

    1. Front Matter
      Pages 249-249
    2. Konstantin Starchev, Kevin Wilkinson, Jacques Buffle
      Pages 251-275
    3. Jerker Widengren
      Pages 276-301
  7. Environmental Analysis and Monitoring

    1. Front Matter
      Pages 303-303
    2. Petra Schwille
      Pages 360-378
    3. Michael Brinkmeier
      Pages 379-395
    4. Joachim D. Müller, Yan Chen, Enrico Gratton
      Pages 410-437
    5. Jennifer L. Mitchell, Nancy L. Thompson
      Pages 438-458
    6. R. Rigler, S. Wennmalm, L. Edman
      Pages 459-476
  8. Back Matter
    Pages 477-489

About this book


This book presents the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. FCS is based on the detection of single molecules excited to fluorescence in diffraction limited confocal volume elements and the time correlation of stochastic events. It provides ultimate sensitivity in the analysis of molecular processes and has found numerous applications in physics, chemistry and particularly in biomolecular sciences. Its high spatial and temporal resolution has made FCS a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion and flow, as well as the physics of the excited state in solution as well as at the cellular level. Its application in high throughput drug screening is using all the potential of this prime analytical tool.


Fluorescence correlation microscopy Single molecule analysis Thermodynamic fluctuations diffraction microscopy molecule optics polarization spectroscopy stochastic phenomena and correlated motion

Authors and affiliations

  • Rudolf Rigler
    • 1
  • Elliot S. Elson
    • 2
  1. 1.Department of Medical BiophysicsKarolinska InstitutetStockholmSweden
  2. 2.Department of Biochemistry and Molecular Biophysics, School of MedicineWashington UniversitySt. LouisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2001
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-64018-6
  • Online ISBN 978-3-642-59542-4
  • Series Print ISSN 0172-6218
  • Buy this book on publisher's site