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Rapid Cycle Real-Time PCR — Methods and Applications

Genetics and Oncology

  • Wolfgang Dietmaier
  • Carl Wittwer
  • Natarajan Sivasubramanian

Table of contents

  1. Front Matter
    Pages I-VIII
  2. Introduction for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications

    1. Wolfgang Dietmaier, Carl Wittwer, Natarajan Sivasubramanian
      Pages 1-2
  3. Methods Useful in Genetics and Oncology

    1. Front Matter
      Pages 3-3
    2. Xi Zhu, Georg Baumgarten, Feng Wang, Ziad Dibbs, Abhinav Diwan, Guillermo Torre-Amione et al.
      Pages 5-14
    3. Danuta Kielar, Wolfgang Dietmaier, Thomas Langmann, Charalampos Aslanidis, Mario Probst, Marek Naruszewicz et al.
      Pages 15-25
    4. Nathalie Pieri-Balandraud, Jean Roudier, Chantal Roudier
      Pages 27-33
  4. Applications in Genetics

    1. Front Matter
      Pages 65-65
    2. Stefan Fronhoffs, Thomas Brüning, Hans Vetter, Yon Ko
      Pages 67-75
    3. Siegfried Burggraf, Naeem Malik, Edith Schuhmacher, Bernhard Olgemöller
      Pages 85-94
    4. Lukas Bestmann, Näder Helmy, Felicia Garofalo, Aynur Demirtas, Dieter Vonderschmitt, Friedrich E. Maly
      Pages 95-105
    5. Tsutomu Aoshima, Mitsuharu Kajita, Yoshitaka Sekido, Shunji Mimura, Kazuyoshi Watanabe, Kaoru Shimokata et al.
      Pages 107-114
  5. Applications in Oncology

    1. Front Matter
      Pages 137-137
    2. Monica L. Henriquez, Brian E. Caplin, Randy P. Rasmussen
      Pages 147-157
    3. Martin Weisser, Claudia Schoch, Torsten Haferlach, Wolfgang Hiddemann, Susanne Schnittger
      Pages 159-168
    4. Jochen Greiner, Mark Ringhoffer, Anita Szmaragowska, Sandra Hübsch, Hartmut Döhner, Michael Schmitt
      Pages 177-186

About this book

Introduction

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier!

Keywords

DNA TNF biopsy cancer cytokine diagnostics gastric cancer gene expression genetics genome protein screening

Editors and affiliations

  • Wolfgang Dietmaier
    • 1
  • Carl Wittwer
    • 2
  • Natarajan Sivasubramanian
    • 3
  1. 1.Institut für PathologieRegensburgGermany
  2. 2.Department of PathologyUniversity of Utah Medical SchoolSalt Lake CityUSA
  3. 3.Baylor College of MedicineHoustonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-59397-0
  • Copyright Information Springer-Verlag Berlin Heidelberg 2002
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-63965-4
  • Online ISBN 978-3-642-59397-0
  • Buy this book on publisher's site