Cell-Free Translation Systems

  • Alexander S. Spirin

Table of contents

  1. Front Matter
    Pages I-VIII
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Alexander S. Spirin
      Pages 3-20
  3. Improved Classical (Batch) Cell-Free Translation Systems

    1. Front Matter
      Pages 21-21
    2. Thorsten Lamla, Volker A. Erdmann
      Pages 23-39
    3. Dong-Myung Kim, James R. Swartz
      Pages 41-52
    4. Takuya Ueda, Akio Inoue, Midori Kaida, Ryoko Baba, Yoshihiro Shimizu
      Pages 53-60
    5. Anatoly T. Gudkov, Kirill A. Martemyanov
      Pages 61-66
    6. Michael Altmann, Hans Trachsel
      Pages 67-78
    7. Giovanna Bergamini, Fátima Gebauer
      Pages 79-88
  4. Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems

    1. Front Matter
      Pages 89-89
    2. Vladimir A. Shirokov, Peter N. Simonenko, Sergey V. Biryukov, Alexander S. Spirin
      Pages 91-107
    3. Kairat Madin, Tatsuya Sawasaki, Yaeta Endo
      Pages 109-119
    4. Bernd Buchberger, Wolfgang Mutter, Albert Röder
      Pages 121-128
  5. Protein Folding in Cell-Free Translation System

    1. Front Matter
      Pages 129-129
    2. Vyacheslav A. Kolb, Aigar Kommer, Alexander S. Spirin
      Pages 131-140
    3. Stacey Traviglia, Monique van Hoek
      Pages 149-156
    4. Cordula Nemetz, Stephanie Wessner, Simone Krupka, Manfred Watzele, Wolfgang Mutter
      Pages 157-163
    5. Robin Steigerwald, Cordula Nemetz, Bärbel Walckhoff, Thomas Emrich
      Pages 165-173
    6. Erhard Fernholz, Katrin Zaiss, Hüseyin Besir, Wolfgang Mutter
      Pages 175-179
  6. Special Topics and Short Communications

    1. Front Matter
      Pages 181-182
    2. Tsutomu Suzuki, Takashi Ohtsuki, Yoh-ichi Watanabe, Maki Terasaki, Takao Hanada, Kimitsuna Watanabe
      Pages 183-196
    3. Vincent Monchois, Renaud Vincentelli, Céline Deregnaucourt, Chantal Abergel, Jean-Michel Claverie
      Pages 197-202
    4. Thomas Hoffmann, Cordula Nemetz, Regina Schweizer, Wolfgang Mutter, Manfred Watzele
      Pages 203-210
    5. Andrea Graentzdoerffer, Manfred Watzele, Bernd Buchberger, Sabine Wizemann, Thomas Metzler, Wolfgang Mutter et al.
      Pages 211-217
    6. Jean-Michel Betton, Nicolae Palibroda, Abdelkader Namane, Thomas Metzler, Octavian Bârzu
      Pages 219-225
    7. Ho S. Cho, Jeffrey G. Pelton, Weiru Wang, Hisao Yokota, David E. Wemmer
      Pages 227-233
    8. Michael Schräml, Dorothee Ambrosius, Jan Stracke, Martin Lanzendörfer
      Pages 235-246
    9. Miyuki Murayama, Xiaoyan Sun, Akihiko Takashima
      Pages 247-252
  7. Back Matter
    Pages 253-259

About this book


This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.


Expression Polymerasekettenreaktion Protein Expression Proteinsynthese RNA bacteria biosynthesis gene expression protein protein synthesis translation yeast

Editors and affiliations

  • Alexander S. Spirin
    • 1
  1. 1.Institute for Protein ResearchRussian Academy of SciencesPushchino, Moscow RegionRussia

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2002
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-63956-2
  • Online ISBN 978-3-642-59379-6
  • Buy this book on publisher's site