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Protein Structure Analysis

Preparation, Characterization, and Microsequencing

  • Roza Maria Kamp
  • Theodora Choli-Papadopoulou
  • Brigitte Wittmann-Liebold

Part of the Springer Lab Manual book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Separation of Proteins

  3. Separation of Peptides

  4. Manual and Automated Microsequencing Methods

  5. Electrophoresis and Blotting of Proteins

    1. Front Matter
      Pages 181-181
    2. P. Jungblut
      Pages 183-214
  6. Analysis of Amino Acids

    1. Front Matter
      Pages 229-229
    2. R. M. Kamp
      Pages 231-248
    3. D. Vollenbroich, K. Krause
      Pages 249-255
  7. Identification of Cysteine Residues and Lipids in Proteins

    1. Front Matter
      Pages 257-257
    2. T. G. Giannakouros
      Pages 269-276
  8. Mass Spectrometry in Peptide and Protein Analysis

  9. Back Matter
    Pages 313-316

About this book

Introduction

The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories; they are easy to perform and thus also suitable for beginners. Enclosed are protocols on the separation of proteins and peptides, manual and automated microsequencing, electrophoresis and blotting, analysis of amino acids, identification of cysteine residues and lipids as well as on mass spectrometry crystallization of macromolecules.

Keywords

Amino acid Blotting Electrophoresis Elektrophorese Mass spectrometry Massenspektrometrie Protein sequencing Proteinreinigung Proteinsequenzierung Proteinstruktur protein structure proteins

Editors and affiliations

  • Roza Maria Kamp
    • 1
  • Theodora Choli-Papadopoulou
    • 2
  • Brigitte Wittmann-Liebold
    • 3
  1. 1.Technische Fachhochschule Berlin FB3 Chemie- und BiotechnikBerlinGermany
  2. 2.School of Biochemistry, Laboratory of BiochemistryAristotle University ThessalonikiThessalonikiGreece
  3. 3.Max-Delbrück-Centrum für Molekulare MedizinBerlin-BuchGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-59219-5
  • Copyright Information Springer-Verlag Berlin Heidelberg 1997
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-47765-2
  • Online ISBN 978-3-642-59219-5
  • Buy this book on publisher's site