High-Dimensional Single Cell Analysis

Mass Cytometry, Multi-parametric Flow Cytometry and Bioinformatic Techniques

  • Harris G. Fienberg
  • Garry P. Nolan

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 377)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jonathan M. Irish, Deon B. Doxie
    Pages 1-21
  3. Nevena Zivanovic, Andrea Jacobs, Bernd Bodenmiller
    Pages 95-109
  4. Björn Koos, Linda Andersson, Carl-Magnus Clausson, Karin Grannas, Axel Klaesson, Gaëlle Cane et al.
    Pages 111-126
  5. Gérald Grégori, Bartek Rajwa, Valery Patsekin, James Jones, Motohiro Furuki, Masanobu Yamamoto et al.
    Pages 191-210
  6. Back Matter
    Pages 211-215

About this book


This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics; and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is  an invaluable reference book for both basic and clinical researchers.


Apoptotic signaling Cancer cell subsets Lymphoma Mass cytometry Multi-parametric flow cytometry Pediatric leukemia Single cell biology T cell phenotype

Editors and affiliations

  • Harris G. Fienberg
    • 1
  • Garry P. Nolan
    • 2
  1. 1.Center for Clinical Sciences Research, Department of Microbiology and ImmunologyStanford School of MedicineStanfordUSA
  2. 2.Center for Clinical Sciences Research, Department of Microbiology and ImmunologyStanford School of MedicineStanfordUSA

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2014
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-3-642-54826-0
  • Online ISBN 978-3-642-54827-7
  • Series Print ISSN 0070-217X
  • Series Online ISSN 2196-9965
  • Buy this book on publisher's site