Molecular Analysis of DNA Rearrangements in the Immune System

  • Rolf Jessberger
  • Michael R. Lieber

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 217)

Table of contents

  1. Front Matter
    Pages I-IX
  2. Dik C. van Gent, J. Fraser McBlane, Dale A. Ramsden, Moshe J. Sadofsky, Joanne E. Hesse, Martin Gellert
    Pages 1-10
  3. David G. Schatz, Thomas M. J. Leu
    Pages 11-29
  4. Ulf Grawunder, Thomas H. Winkler, Fritz Melchers
    Pages 31-43
  5. Stephen Desiderio, Weei-Chin Lin, Zhong Li
    Pages 45-59
  6. Sharri Bockheim Steen, Chengming Zhu, David B. Roth
    Pages 61-77
  7. Carl W. Anderson, Timothy H. Carter
    Pages 91-111
  8. Rolf Jessberger, Matthias Wabl, Tilman Borggrefe
    Pages 191-202
  9. Matthias Wabl, Charles Steinberg
    Pages 203-219
  10. Back Matter
    Pages 221-224

About this book

Introduction

The vertebrate immune system is distinctive among defense systems of multicellular organisms. In addition to nonspecific immunity, it generates a randomized array of millions of antigen receptors (immunoglobulins and T-cell receptors). A subset of these receptors are critical for binding to invading microbes or biochemicals from them to tag the microbes for elimination. Three site-directed DNA modification processes are critical to this process in vertebrates. V(D)J recombination generates the array of exons that encode the antigen binding pockets. Recent work summarized in this volume describes the dissection of this process at the biochemical level. The mechanism of the reaction is now understood in considerable detail. The proteins that catalyze many steps of the process have now been identified by biochemical and genetic recon­ stitution and by analysis of genetic mutants defective in V(D)J recombination. Class switch recombination is the process by which the variable domain exon of the heavy chain is changed from IgM to IgG, IgA. or IgE. Recent progress is described in the de­ velopment of an extrachromosomal substrate assay system. Molecular genetic analysis of the process in transgenics is defining some of the cis sequence requirements. Biochemical assays for defining enzymatic components are also described. In addition to exciting progress in V(D)J recombination and class switch recombination, one chapter describes recent pro­ gress in somatic hypermutation.

Keywords

DNA antigen biochemistry cell cell cycle gene gene expression immune system immunoglobulin mutation protein proteins receptor regulation transcription

Editors and affiliations

  • Rolf Jessberger
    • 1
  • Michael R. Lieber
    • 2
  1. 1.Institute for ImmunologyBaselSwitzerland
  2. 2.Division of Molecular Oncology, Department of PathologyWashington University School of MedicineSt. LouisUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-50140-1
  • Copyright Information Springer-Verlag Berlin Heidelberg 1996
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-642-50142-5
  • Online ISBN 978-3-642-50140-1
  • Series Print ISSN 0070-217X
  • About this book