Methods in Endothelial Cell Biology

  • Hellmut G. Augustin

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XX
  2. Isolation and Culture of Endothelial Cells

    1. Front Matter
      Pages 1-1
    2. Ralf P. Brandes, Stefani Beer, Alexandra Aicher
      Pages 15-22
    3. Jun Yamashita, Shin-Ichi Nishikawa
      Pages 33-45
    4. Taija Mäkinen, Kari Alitalo
      Pages 47-54
    5. Yvonne Reiss, Friedemann Kiefer
      Pages 63-72
    6. Christoph Volpers, Stefan Kochanek
      Pages 73-82
  3. Functional Assays in Vitro

    1. Front Matter
      Pages 83-83
    2. Olaf Zilles, Markus Hammel, Rupert Hallmann
      Pages 85-91
    3. Andreas Schubert, Hennig Morawietz
      Pages 93-101
    4. Maria Grazia Lampugnani, Elisabetta Dejana
      Pages 103-113
    5. Roberto F. Nicosia, Wen-Hui Zhu
      Pages 125-144
    6. Hellmut G. Augustin
      Pages 145-156
    7. Yvonne Reiss, Britta Engelhardt
      Pages 157-165
    8. Sung-Suk Chae, Ji-Hye Paik, Jonathan Shubert-Coleman, Henry Furneaux, Timothy Hla
      Pages 167-176
  4. Functional Assays in Vivo

    1. Front Matter
      Pages 177-177
    2. Toshinori Murayama, Oren M. Tepper, Takayuki Asahara
      Pages 179-185
    3. Volkhard Lindner
      Pages 187-195
    4. Stephan Schirmer, Imo Hoefer, Ivo Buschmann
      Pages 197-206
    5. Tony Passaniti, Michele I. Vitolo
      Pages 207-222
    6. Katja Issbrücker, Hugo H. Marti, Matthias Clauss
      Pages 223-232
    7. John S. Rudge, Gavin Thurston
      Pages 233-245
    8. Lucia Morbidelli, Silvia Cantara, Marina Ziche
      Pages 263-272
  5. Detection of Endothelial Cells

    1. Front Matter
      Pages 273-273
    2. Cynthia Obodozie, Jens Kroll
      Pages 275-286
    3. Regina Heidenreich, Georg Breier
      Pages 301-312
    4. Holger Gerhardt, Christer Betsholtz
      Pages 313-323
    5. Jody J. Haigh, Andras Nagy
      Pages 325-336
    6. Mohammad Farhadi, Karsten Schwinn, Peter Vajkoczy
      Pages 347-355
    7. Valentin Djonov, Peter H. Burri
      Pages 357-369
  6. Back Matter
    Pages 371-383

About this book


Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.

Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.


Angiogenesis Cell culture Vascular Biology Vivo blood vessel bone cell biology endothelium gene transfer hypertension leukocyte

Editors and affiliations

  • Hellmut G. Augustin
    • 1
  1. 1.Department of Vascular Biology & Angiogenesis ResearchTumor Biology Center at the University of FreiburgFreiburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2004
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Book Archive
  • Print ISBN 978-3-540-21397-0
  • Online ISBN 978-3-642-18725-4
  • Buy this book on publisher's site