MicroRNA Expression Detection Methods

  • Zhiguo Wang
  • Baofeng Yang

Table of contents

  1. Front Matter
    Pages i-xx
  2. General Remarks

    1. Front Matter
      Pages 1-1
  3. miRNA Microarray Methods

    1. Front Matter
      Pages 66-66
    2. Zhiguo Wang, Baofeng Yang
      Pages 67-79
  4. Northern Blotting Methods

  5. In Situ Hybridization Methods

  6. Real-Time RT-PCR Methods

    1. Front Matter
      Pages 130-130
    2. Zhiguo Wang, Baofeng Yang
      Pages 131-140
    3. Zhiguo Wang, Baofeng Yang
      Pages 141-146
    4. Zhiguo Wang, Baofeng Yang
      Pages 147-151
    5. Zhiguo Wang, Baofeng Yang
      Pages 153-157
    6. Zhiguo Wang, Baofeng Yang
      Pages 159-170
  7. Cloning Methods

    1. Front Matter
      Pages 172-172
    2. Zhiguo Wang, Baofeng Yang
      Pages 173-188
  8. Nanoparticle Methods

About this book

Introduction

This book provides comprehensive descriptions of the innovative strategies and methodologies for detecting miRNA expression, their application in miRNA research and their potential as tools for clinical diagnosis and prognosis.

It begins with the introduction of the overall concept and strategies of miRNA expression detection methods emphasizing the need of a wide variety of these methods to suit specific requirements for research and clinical examination in laboratories. In the following, each single chapter focuses on an independent, unique method of miRNA detection and is divided into five subsections: summary, introduction, protocol (including materials, instrument, reagent, and procedure), application and limitation, and reference. The development of the technique, the ideas behind it and the mechanisms underlying the method are given in the introduction of each chapter. The step-by-step protocols are detailed in the protocol section. Then, the applications and limitations of the methods are discussed. Finally, the literature citations are listed in reference section. Schematic diagrams are included where needed and appropriate for better illustrating the principle of the methodologies. In addition, flowcharts are also provided to outline the protocols for each of the miRNA expression detection methods.

This book is aimed to target both researchers involved in miRNA research of any fields in universities and research institutions and medical practitioners who are interested in developing or utilizing miRNA profiling as a complementary and an alternative strategy for clinical diagnosis of human disease.

Keywords

FISH Ligation Microarray PCR gene expression gene therapy genes hybridization miRNA miRNA detection miRNA expression miRNA profiling miRNAs microRNA transcription

Authors and affiliations

  • Zhiguo Wang
    • 1
  • Baofeng Yang
    • 2
  1. 1.Research CenterMontreal Heart InstituteMontrealCanada
  2. 2.Dept. PharmacologyHarbin Medical UniversityHarbinChina, People's Republic

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-642-04928-6
  • Copyright Information Springer-Verlag Berlin Heidelberg 2010
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-3-642-04927-9
  • Online ISBN 978-3-642-04928-6
  • About this book