Antibody Engineering

  • Roland Kontermann
  • Stefan Dübel

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Bioinformatics of Antigen-binding Sites

    1. Front Matter
      Pages 1-1
    2. Svetlana Mollova, Ida Retter, Michael Hust, Stefan Dübel, Werner Müller
      Pages 3-10
    3. François Ehrenmann, Patrice Duroux, Véronique Giudicelli, Marie-Paule Lefranc
      Pages 11-31
  3. Generation of Antibody Fragments and Their Derivatives

    1. Front Matter
      Pages 54-54
    2. Dafne Müller
      Pages 55-59
    3. Roland E. Kontermann
      Pages 61-68
    4. Jonas V. Schaefer, Peter Lindner, Andreas Plückthun
      Pages 85-99
    5. Athanasios Mavratzas, Evelyn Exner, Jürgen Krauss, Michaela A. E. Arndt
      Pages 101-112
    6. Dafne Müller, Jeannette Gerspach
      Pages 113-126
    7. Ira Pastan, Mitchell Ho
      Pages 127-146
    8. Bianca Altvater, Silke Landmeier, Claudia Rossig
      Pages 147-159
    9. Alessio Cardinale, Silvia Biocca
      Pages 161-172
    10. Nina Strebe, Manuela Schüngel
      Pages 173-180
    11. Ulrich Brinkmann
      Pages 181-189
    12. Arutselvan Natarajan, Sally J. DeNardo
      Pages 191-205
    13. Roland Stork
      Pages 207-218
    14. Laila Al-Halabi, Torsten Meyer
      Pages 219-226

About this book


Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity.

Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.


Antigen Cancer therapy Hybridoma Immunoglobulins Immunology Protein engineering imaging

Editors and affiliations

  • Roland Kontermann
    • 1
  • Stefan Dübel
    • 2
  1. 1.(Biomedical Engineering) Institut für Zellbiologie und ImmunologieUniversität StuttgartStuttgartGermany
  2. 2.Technische Universität BraunschweigInstitut für Biochemie und BiotechnologieGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2010
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-642-01146-7
  • Online ISBN 978-3-642-01147-4
  • About this book