Antibody Engineering

  • Roland Kontermann
  • Stefan Dübel

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Cloning of the Antigen-binding Site from Hybridoma

    1. Front Matter
      Pages 1-1
    2. Nina Strebe, Frank Breitling, Dieter Moosmayer, Bodo Brocks, Stefan Dübel
      Pages 3-14
    3. Andrew Bradbury
      Pages 15-20
    4. Jonas V. Schaefer, Annemarie Honegger, Andreas Plückthun
      Pages 21-44
  3. Generation of Antibody Repertoires

    1. Front Matter
      Pages 43-43
    2. Christian Kellner, Sahar Mohseni Nodehi, Matthias Peipp
      Pages 47-63
    3. Michael Hust, Stefan Dübel
      Pages 65-84
    4. Pierre Martineau
      Pages 85-97
    5. Thibaut Pelat, Michael Hust, Philippe Thullier
      Pages 99-114
    6. Rüdiger Ridder, Hermann Gram
      Pages 115-123
  4. Selection of Antibody Fragments from Combinatorial Libraries

    1. Front Matter
      Pages 128-128
    2. Roland E. Kontermann
      Pages 127-137
    3. Michael Hust, Michael Mersmann
      Pages 139-149
    4. Patrick Chames, Daniel Baty
      Pages 151-164
    5. Steffen U. Eisenhardt, Karlheinz Peter
      Pages 165-182
    6. Frank Breitling, Olaf Broders, Saskia Helmsing, Michael Hust, Stefan Dübel
      Pages 197-206
    7. Kelly Davis Orcutt, K. Dane Wittrup
      Pages 207-233
    8. Xiangang Zou, Guocheng Lan, Michael J. Osborn, Marianne Brüggemann
      Pages 235-254
    9. Sabine Rauth, Martin Schlapschy, Arne Skerra
      Pages 255-266
    10. Zoltán Konthur, Jeannine Wilde, Theam Soon Lim
      Pages 267-287
  5. Engineering and Production of Immunoglobulins

    1. Front Matter
      Pages 290-290
    2. Zehra Kaymakcalan, Alexander Ibraghimov, Andrew G. Goodearl, Jochen G. Salfeld
      Pages 291-306
    3. David Gareth Williams, David J. Matthews, Tarran Jones
      Pages 319-339
    4. Johan Desmet, Karen Vanhoorelbeke, Hans Deckmyn
      Pages 341-353
    5. Sang Jick Kim, Insoo Park, Hyo Jeong Hong
      Pages 355-368
    6. Philippe Alard, Johan Desmet, Ignace Lasters
      Pages 369-376
    7. Vania E. Kenanova, Tove Olafsen, Jan T. Andersen, Inger Sandlie, Anna M. Wu
      Pages 411-430
    8. Richard Begent, Surinder Sharma, Kerry Chester
      Pages 431-451
    9. Thomas Beyer, Stefan Lohse, Michael Dechant, Thomas Valerius
      Pages 471-486
  6. Antibody Characterization

    1. Front Matter
      Pages 488-488
    2. Doreen M. Floss, Udo Conrad
      Pages 489-502
    3. Thomas Jostock, Jiandong Li
      Pages 517-529
    4. Kirstin A. Zettlitz
      Pages 531-535
    5. Frank Breitling, Christopher Schirwitz, Thomas Felgenhauer, Ines Block, Volker Stadler, Ralf Bischoff
      Pages 573-589
    6. Consuelo Garcia-Rodriguez, Yu Zhou, James D. Marks
      Pages 591-605
    7. Bernd Voedisch, Holger Thie
      Pages 607-612
    8. Marie-Claire Janin-Bussat, Jean-Marc Strub, Elsa Wagner-Rousset, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaia et al.
      Pages 613-634
    9. Marie-Claire Janin-Bussat, Elsa Wagner-Rousset, Christine Klinguer-Hamour, Nathalie Corvaia, Alain van Dorsselaer, Alain Beck
      Pages 635-656
    10. Pierre Martineau
      Pages 657-665
    11. Verena Boschert, Peter Scheurich
      Pages 695-704

About this book


Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity.

Antibody Engineering provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest "hands on" knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.


Antigen Cancer therapy Chloroplast Expression Hybridoma Immunoglobulins Immunology Polymerasekettenreaktion Protein engineering chromatography cloning diagnosis mutagenesis protein transgen

Editors and affiliations

  • Roland Kontermann
    • 1
  • Stefan Dübel
    • 2
  1. 1.(Biomedical Engineering) Institut für Zellbiologie und ImmunologieUniversität StuttgartStuttgartGermany
  2. 2.Technische Universität BraunschweigInstitut für Biochemie und BiotechnologieGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag Berlin Heidelberg 2010
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-642-01143-6
  • Online ISBN 978-3-642-01144-3
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site