Imaging Cellular and Molecular Biological Functions

Editors:

ISBN: 978-3-540-71330-2 (Print) 978-3-540-71331-9 (Online)

Table of contents (16 chapters)

  1. Front Matter

    Pages i-xxi

  2. Considerations for Routine Imaging

    1. Front Matter

      Pages 2-2

    2. No Access

      Chapter

      Pages 3-43

      Entering the Portal: Understanding the Digital Image Recorded Through a Microscope

    3. No Access

      Chapter

      Pages 45-70

      Quantitative Biological Image Analysis

    4. No Access

      Chapter

      Pages 71-92

      The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics

    5. No Access

      Chapter

      Pages 93-113

      Design and Function of a Light-Microscopy Facility

  3. Advanced Methods and Concepts

    1. Front Matter

      Pages 116-116

    2. No Access

      Chapter

      Pages 117-155

      Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls

    3. No Access

      Chapter

      Pages 157-181

      Quantitative FRET Microscopy of Live Cells

    4. No Access

      Chapter

      Pages 183-233

      Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells

    5. No Access

      Chapter

      Pages 235-263

      Single Fluorescent Molecule Tracking in Live Cells

    6. No Access

      Chapter

      Pages 265-285

      From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins

  4. Cutting-Edge Applications and Utilities

    1. Front Matter

      Pages 288-288

    2. No Access

      Chapter

      Pages 289-312

      Towards Imaging the Dynamics of Protein Signalling

    3. No Access

      Chapter

      Pages 313-343

      New Technologies for Imaging and Analysis of Individual Microbial Cells

    4. No Access

      Chapter

      Pages 345-364

      Imaging Parasites in Vivo

    5. No Access

      Chapter

      Pages 365-384

      Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells

    6. No Access

      Chapter

      Pages 385-405

      High-Throughput/High-Content Automated Image Acquisition and Analysis

    7. No Access

      Chapter

      Pages 407-422

      Cognition Network Technology – A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents

    8. No Access

      Chapter

      Pages 423-442

      High-Content Phenotypic Cell-Based Assays

  5. Back Matter

    Pages 443-449