Fluorescence In Situ Hybridization (FISH) — Application Guide

  • Thomas Liehr

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Molecular Cytogenetics, Probes and Equipment

    1. Thomas Liehr, Franck Pellestor
      Pages 23-34
    2. Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, Nigel P. Carter
      Pages 35-52
    3. Anja Weise, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Madeleine Gross, Vivien Klaschka et al.
      Pages 53-60
    4. Franck Pellestor, Petra Paulasova, Samir Hamamah
      Pages 61-66
    5. Sean Bradley, Leah Zamechek, Joan Aurich-Costa
      Pages 67-73
    6. Ivan Y. Iourov
      Pages 75-84
    7. Michael Sommerauer, Ingrid Feuerbacher
      Pages 85-97
    8. Vladimir A. Trifonov, Nadezhda N. Vorobieva, Willem Rens
      Pages 99-109
  3. FISH Applications in Different Tissue Preparations

    1. Bernd Eiben, Ralf Glaubitz, Arens Hans-Jürgen
      Pages 123-130
    2. Anita Heller, Madeleine Gross, Vera Witthuhn, Katja Behr, Sophie Hinreiner, Hasmik Mkrtchyan et al.
      Pages 131-136
    3. Thomas Liehr, Erich Gebhart
      Pages 137-140
    4. Kristin Mrasek, Anja Weise, Madeleine Gross, Hasmik Mkrtchyan, Thomas Liehr
      Pages 141-146
    5. Maria Oliver-Bonet
      Pages 157-172
  4. Characterization of Marker Chromosomes

    1. Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Joana Melo, Anna Polityko et al.
      Pages 175-181
    2. Karin M. Greulich-Bode, Maren Kaufmann, Klaus Gilbertz, Roderick A. F. MacLeod
      Pages 183-192
    3. Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye et al.
      Pages 193-216

About this book

Introduction

This FISH application guide provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are successfully used to study many aspects of genomic behavior and alterations. In 36 chapters, contributed by international experts in their particular field, the nowadays multiple approaches and applications of the powerful techniques are presented and detailed protocols are given.

Described here are methods using various cell types and tissues as well as different organisms, such as mammalians, insects, plants and microorganisms. Multicolor FISH procedures and special applications such as the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear architecture and epigenetic changes, as well as (array-based) comparative genomic hybridization studies are presented. Overall, the technique of choice is introduced for single cell analysis in human genetics, microbiology, animal and plant sciences.

Keywords

Chromosom Comparative genomic hybridization Cytogenetics DNA Epigenetic aberrations Fluoreszenz in situ-Hybridisierung Interphase Nuclear architecture PRINS Termination bone genetics hybridization microscopy tissue

Editors and affiliations

  • Thomas Liehr
    • 1
  1. 1.Institut für Humangenetik und AnthropologieJenaGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-540-70581-9
  • Copyright Information Springer-Verlag Berlin Heidelberg 2009
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Springer Protocols
  • Print ISBN 978-3-540-70580-2
  • Online ISBN 978-3-540-70581-9
  • About this book