Advertisement

Mammalian and Avian Transgenesis — New Approaches

  • Shirley Pease
  • Carlos Lois

Part of the Principles and Practice book series (PRINCIPLES)

Table of contents

  1. Front Matter
    Pages I-XX
  2. Ming-Wen Li, K. C. Kent Lloyd
    Pages 23-40
  3. Kevin Eggan, Rudolf Jaenisch
    Pages 69-96
  4. Shiaoching Gong, Nat Heintz
    Pages 97-110
  5. Mansuo L. Hayashi, Shigemi Hayashi
    Pages 111-130
  6. William A. Ritchie
    Pages 173-193
  7. Robert M. Petters, Rebecca L. Krisher
    Pages 195-220
  8. Benjamin B. Scott, Carlos Lois
    Pages 221-230
  9. Shirley Pease
    Pages 231-276
  10. Back Matter
    Pages 277-281

About this book

Introduction

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.

The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Keywords

DNA Lentivirus Tempo cloning gene expression gene transfer genes mutation nuclear transfer transgenesis transgenic animals

Editors and affiliations

  • Shirley Pease
    • 1
  • Carlos Lois
    • 2
  1. 1.Division of Biology, 147-75California Institute of TechnologyPasadenaUSA
  2. 2.Massachusetts Institute of Technology, E25-421CambridgeUSA

Bibliographic information