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Imaging, Manipulation and Optogenetics in Zebrafish

  • Itia Amandine Favre‐Bulle

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Itia Amandine Favre-Bulle
    Pages 1-7
  3. Itia Amandine Favre-Bulle
    Pages 9-20
  4. Itia Amandine Favre-Bulle
    Pages 21-31
  5. Itia Amandine Favre-Bulle
    Pages 33-47
  6. Itia Amandine Favre-Bulle
    Pages 63-84
  7. Itia Amandine Favre-Bulle
    Pages 85-86

About this book

Introduction

The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.

Keywords

In vivo Micromanipulation Optogenetics Zebrafish Neurobiology Optical Micromanipulation Light Sheet Microscopy Laser Light for Neuroscience Optical Properties of Otoliths Optical Trapping

Authors and affiliations

  • Itia Amandine Favre‐Bulle
    • 1
  1. 1.School of Mathematics and PhysicsThe University of QueenslandBrisbaneAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-96250-4
  • Copyright Information Springer Nature Switzerland AG 2018
  • Publisher Name Springer, Cham
  • eBook Packages Physics and Astronomy
  • Print ISBN 978-3-319-96249-8
  • Online ISBN 978-3-319-96250-4
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site