Human Neural Stem Cells

From Generation to Differentiation and Application

  • Leonora┬áBuzanska

Part of the Results and Problems in Cell Differentiation book series (RESULTS, volume 66)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Derivation and/or Generation of Human Neural Stem Cells

    1. Front Matter
      Pages 1-1
    2. David J. Eve, Paul R. Sanberg, Leonora Buzanska, Anna Sarnowska, Krystyna Domanska-Janik
      Pages 21-87
    3. Daekee Kwon, Hee-Jin Ahn, Kyung-Sun Kang
      Pages 103-121
  3. Differentiation and Underlying Mechanisms

    1. Front Matter
      Pages 123-123
    2. Mizuki Honda, Kinichi Nakashima, Sayako Katada
      Pages 125-136
    3. Sridhar T. Narla, Brandon Decker, Pinaki Sarder, Ewa K. Stachowiak, Michal K. Stachowiak
      Pages 137-162
    4. Raul Bardini Bressan, Steven M. Pollard
      Pages 163-182
    5. L. B. Chuye, A. Dimitri, A. Desai, C. Handelmann, Y. Bae, P. Johari et al.
      Pages 183-206
  4. Therapeutic Potential of Human Neural Stem Cells

    1. Front Matter
      Pages 231-231
    2. Yang D. Teng, Lei Wang, Xiang Zeng, Liquan Wu, Zafer Toktas, Serdar Kabatas et al.
      Pages 233-248
    3. Zaal Kokaia, Vladimer Darsalia
      Pages 249-263
    4. Cameron D. Pernia, Neal H. Nathan, Brian T. D. Tobe, Alicia M. Winquist, Richard L. Sidman, Yoshio Goshima et al.
      Pages 265-282
    5. Ewa Liszewska, Jacek Jaworski
      Pages 283-305
    6. Daniela Ferrari, Maurizio Gelati, Daniela Celeste Profico, Angelo Luigi Vescovi
      Pages 307-329

About this book


This book summarizes early pioneering achievements in the field of human neural stem cell (hNSC) research and combines them with the latest advances in stem cell technology, including reprogramming and gene editing. The powerful potential of hNSC to generate and repair the developing and adult CNS has been confirmed by numerous experimental in vitro and in vivo studies. The book presents methods for hNSC derivation and discusses the mechanisms underlying NSC in vitro fate decisions and their in vivo therapeutic mode of action.

The long-standing dogma that the human central nervous system (CNS) lacks the ability to regenerate was refuted at the end of the 20th century, when evidence of the presence of neurogenic zones in the adult human brain was found. These neurogenic zones are home to human neural stem cells (hNSCs), which are capable of self-renewing and differentiating into neurons, astrocytes and oligodendrocytes. NSCs isolated from human CNS have a number of clinical advantages, especially the innate potential to differentiate into functional neural cells. Nevertheless, their full clinical exploitation has been hindered by limited access to the tissue and low expansion potential. The search for an alternative to CNS sources of autologous, therapeutically competent hNSCs was the driving force for the many studies proving the in vitro plasticity of different somatic stem cells to generate NSCs and their functional progeny. Now the era of induced pluripotent stem cells has opened entirely new opportunities to achieve research and therapeutic goals with the aid of hNSCs.


human neural stem cells hNSC hNSC derivation hNSC differentiation hNSC engineering hNSC clinical application

Editors and affiliations

  • Leonora┬áBuzanska
    • 1
  1. 1.Stem Cell Bioengineering Unit, Mossakowski Medical Research CentrePolish Academy of SciencesWarsawPoland

Bibliographic information