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Fluorescent Tools for Imaging Oxidative Stress in Biology

  • Amandeep┬áKaur

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xlv
  2. Amandeep Kaur
    Pages 1-36
  3. Amandeep Kaur
    Pages 37-57
  4. Amandeep Kaur
    Pages 59-91
  5. Amandeep Kaur
    Pages 113-128
  6. Amandeep Kaur
    Pages 129-147
  7. Amandeep Kaur
    Pages 149-169
  8. Amandeep Kaur
    Pages 171-182
  9. Amandeep Kaur
    Pages 183-188
  10. Amandeep Kaur
    Pages 189-223
  11. Back Matter
    Pages 225-230

About this book

Introduction

This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases.

The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies.

In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research.

Keywords

Ratiometric Fluorescent Probes Oxidative Stress Flavin-based Redox Probes Nicotinamide-based Redox Probes FRET-based Redox Probes Biological Imaging Cellular Redox State Embryonic Stem Cells Pancreatic Cancer Cells Ex Vivo Studies

Authors and affiliations

  • Amandeep┬áKaur
    • 1
  1. 1.School of ChemistryUniversity of SydneySydneyAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-73405-7
  • Copyright Information Springer International Publishing AG 2018
  • Publisher Name Springer, Cham
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-319-73404-0
  • Online ISBN 978-3-319-73405-7
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site