© 2018

A Clinician's Guide to Sperm DNA and Chromatin Damage

  • Armand Zini
  • Ashok Agarwal

Table of contents

  1. Front Matter
    Pages i-xix
  2. Human Sperm Chromatin Structure

    1. Front Matter
      Pages 1-1
    2. Rod Balhorn
      Pages 3-30
    3. Ferran Barrachina, Ada Soler-Ventura, Rafael Oliva, Meritxell Jodar
      Pages 31-51
    4. W. Steven Ward
      Pages 53-61
  3. Laboratory Evaluation of Sperm Chromatin and DNA Damage

    1. Front Matter
      Pages 63-63
    2. Monica Muratori, Elisabetta Baldi
      Pages 91-102
    3. Rakesh Sharma, Zeynep Cakar, Ashok Agarwal
      Pages 103-118
    4. Elva I. Cortés-Gutiérrez, Martha I. Dávila-Rodríguez, Carmen López-Fernández
      Pages 119-135
    5. José Luis Fernández, Stephen Johnston, Jaime Gosálvez
      Pages 137-152
    6. Juris Erenpreiss, Ksenija Zubkova
      Pages 153-162
    7. Michelle M. Kim, Cigdem Tanrikut
      Pages 163-176
  4. Etiology of Sperm DNA Damage: Biological and Clinical Factors

    1. Front Matter
      Pages 177-177
    2. Ralf Henkel, Michael Solomon
      Pages 179-195
    3. Tiphanie Cavé, Olivier Simard, Marie-Chantal Grégoire, Guylain Boissonneault
      Pages 219-227
    4. Rakesh Sharma, Ashok Agarwal
      Pages 229-261
    5. Sarah K. Fatool, Avi Harlev
      Pages 263-279
    6. Peter T. K. Chan, Bernard Robaire
      Pages 281-300

About this book


This comprehensive, up-to-date text, which brings together the key practical elements of the rapidly evolving field of sperm DNA and chromatin abnormalities, is divided thematically into five main sections. Part I discusses human sperm chromatin structure and nuclear architecture, while part II presents laboratory evaluation of sperm DNA damage, including SCSA, SCD, TUNEL and Comet assays, and cytochemical tests. Biological and clinical factors in the etiology of sperm DNA damage are discussed in part III, including oxidative stress, abortive apoptosis, cancer, and environmental and lifestyle factors. Part IV presents clinical studies on the utility of sperm DNA damage tests, both with natural and ART-assisted pregnancies, and debates the clinical utility of such tests. Finally, part V discusses current treatment options, such as antioxidant therapy, varicocelectomy, advanced sperm processing techniques and the use of testicular sperm.

We are now beginning to better understand the unique organization of the sperm chromatin, as well as the nature and etiology of sperm DNA damage. Written and edited by worldwide experts in andrology, A Clinician's Guide to Sperm DNA and Chromatin Damage is an excellent resource for reproductive medicine and REI specialists, urologists, reproductive biologists and any professional working with the infertile male 


Abortive apoptosis Antioxidant therapy Azoospermia Comet assay Cryopreservation Erectile dysfunction Gonadotropin ICSI Male infertility Oxidative stress PICSI-HA binding SCD assay SCS assay Semen analysis Sperm chromatin Sperm DNA damage Sperm nucleoproteins Testicular dysfunction TUNEL assay Varicocelectomy

Editors and affiliations

  • Armand Zini
    • 1
  • Ashok Agarwal
    • 2
  1. 1.Department of Surgery, Division of UrologyMcGill UniversityMontrealCanada
  2. 2.American Center for Reproductive MedicineGlickman Urological and Kidney Institute, Cleveland ClinicClevelandUSA

About the editors

Armand Zini, MD, FRCSC, Professor, Department of Surgery, Division of Urology, St. Mary’s Hospital Center, Montreal, Québec, Canada

Ashok Agarwal, PhD, HCLD, Professor, Lerner College of Medicine of Case Western Reserve University, Director, Andrology Center, Director, American Center for Reproductive Medicine, Department of Urology, Cleveland Clinic Foundation, Cleveland, Ohio, USA

Bibliographic information

  • Book Title A Clinician's Guide to Sperm DNA and Chromatin Damage
  • Editors Armand Zini
    Ashok Agarwal
  • DOI
  • Copyright Information Springer International Publishing AG 2018
  • Publisher Name Springer, Cham
  • eBook Packages Medicine Medicine (R0)
  • Hardcover ISBN 978-3-319-71814-9
  • Softcover ISBN 978-3-030-10116-9
  • eBook ISBN 978-3-319-71815-6
  • Edition Number 1
  • Number of Pages XIX, 605
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Reproductive Medicine
  • Buy this book on publisher's site