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Single-Molecule Metal-Induced Energy Transfer

From Basics to Applications

  • Narain Karedla

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Narain Karedla
    Pages 1-16
  3. Narain Karedla
    Pages 17-74
  4. Narain Karedla
    Pages 87-143
  5. Narain Karedla
    Pages 145-158
  6. Narain Karedla
    Pages 159-160
  7. Back Matter
    Pages 161-166

About this book

Introduction

This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.

Keywords

Superresolution Microscopy Axial Resolution Förster Resonance Energy Transfer Transition Dipole Pattern Matching Fluorescence Lifetime Imaging Microscopy Single-molecule Imaging Defocused Imaging Single-molecule Localization Microscopy

Authors and affiliations

  • Narain Karedla
    • 1
  1. 1.Third Institute of Physics—BiophysicsGöttingen UniversityGöttingenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-60537-1
  • Copyright Information Springer International Publishing AG 2017
  • Publisher Name Springer, Cham
  • eBook Packages Physics and Astronomy
  • Print ISBN 978-3-319-60536-4
  • Online ISBN 978-3-319-60537-1
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
  • Buy this book on publisher's site