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Decoding the Antibody Repertoire

High Throughput Sequencing of Multiple Transcripts from Single B Cells

  • Brandon DeKosky

Part of the Springer Theses book series (Springer Theses)

About this book

Introduction

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.

Keywords

B Cell Single-cell Sequencing Antibody Immunology Immune Repertoire High-throughput Sequencing Next Generation Sequencing B Cell Receptor Antibody Discovery

Authors and affiliations

  • Brandon DeKosky
    • 1
  1. 1.Department of Chemical and Petroleum Engineering, Department of Pharmaceutical ChemistryThe University of KansasLawrenceUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-58518-5
  • Copyright Information Springer International Publishing AG 2017
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-3-319-58517-8
  • Online ISBN 978-3-319-58518-5
  • Series Print ISSN 2190-5053
  • Series Online ISSN 2190-5061
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