Directed Enzyme Evolution: Advances and Applications

  • Miguel Alcalde

Table of contents

  1. Front Matter
    Pages i-vii
  2. Stefan Lutz, Elsie Williams, Pravin Muthu
    Pages 17-67
  3. Robert H. Wilson, Spencer M. Whitney
    Pages 101-126
  4. Patricia Molina-Espeja, Patricia Gómez de Santos, Miguel Alcalde
    Pages 127-143
  5. Amol V. Shivange, Ulrich Schwaneberg
    Pages 145-172
  6. Tong Si, Jiazhang Lian, Huimin Zhao
    Pages 173-200
  7. Kang Lan Tee, Tuck Seng Wong
    Pages 201-227
  8. Valeria A. Risso, Jose M. Sanchez-Ruiz
    Pages 229-255
  9. Emanuele Monza, Sandra Acebes, M. Fátima Lucas, Victor Guallar
    Pages 257-284

About this book


This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art.

Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature.

The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the book summarizes a variety of the most applicable methods for library creation, together with the future trends aimed at bringing together directed evolution and in silico/computational enzyme design and ancestral resurrection.


biocatalysis biomolecular screening industrial biotechnology protein engineering semi-rational approach synthetic biology

Editors and affiliations

  • Miguel Alcalde
    • 1
  1. 1.Department of BiocatalysisInstitute of Catalysis and Petrochemistry, CSICMadridSpain

Bibliographic information