Table of contents

  1. Front Matter
    Pages i-xx
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Joanna Jankowicz-Cieslak, Chikelu Mba, Bradley J. Till
      Pages 3-18 Open Access
  3. Mutation Induction and Chimera Dissociation

    1. Front Matter
      Pages 19-19
    2. Fatemeh Maghuly, Souleymane Bado, Joanna Jankowicz-Cieslak, Margit Laimer
      Pages 21-38 Open Access
    3. Joanna Jankowicz-Cieslak, Bradley J. Till
      Pages 39-54 Open Access
    4. Jorge López, Aymé Rayas, Arletys Santos, Víctor Medero, Yoel Beovides, Milagros Basail
      Pages 55-71 Open Access
    5. Kenneth E. Danso, Wilfred Elegba
      Pages 73-89 Open Access
    6. Iwona Szarejko, Miriam Szurman-Zubrzycka, Malgorzata Nawrot, Marek Marzec, Damian Gruszka, Marzena Kurowska et al.
      Pages 91-111 Open Access
    7. Maia Gurushidze, Stefan Hiekel, Ingrid Otto, Götz Hensel, Jochen Kumlehn
      Pages 113-128 Open Access
    8. Maia Gurushidze, Hannes Trautwein, Petra Hoffmeister, Ingrid Otto, Andrea Müller, Jochen Kumlehn
      Pages 129-141 Open Access
  4. Phenotypic Screening

    1. Front Matter
      Pages 143-143
    2. Sydney D. Johnson, Dennis R. Taylor, Thomas H. Tai, Joanna Jankowicz-Cieslak, Bradley J. Till, Alpha B. Jalloh
      Pages 145-156 Open Access
    3. Michal Slota, Miroslaw Maluszynski, Iwona Szarejko
      Pages 157-172 Open Access
    4. Phuong Tan Tran, Cua Quang Ho
      Pages 173-191 Open Access
    5. Johann Vollmann, Joanna Jankowicz-Cieslak
      Pages 193-202 Open Access
    6. Fatemeh Maghuly, Gorji Marzban, Ebrahim Razzazi-Fazeli, Margit Laimer
      Pages 203-223 Open Access
  5. Genotypic Screening

    1. Front Matter
      Pages 225-225
    2. Owen A. Huynh, Joanna Jankowicz-Cieslak, Banumaty Saraye, Bernhard Hofinger, Bradley J. Till
      Pages 227-239 Open Access
    3. Bernhard J. Hofinger, Owen A. Huynh, Joanna Jankowicz-Cieslak, Bradley J. Till
      Pages 241-251 Open Access

About this book


This book is open access under a CC BY-NC 2.5 license.

This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.


TILLING Embryonic plant cells Mutation detection Plant mutagenesis Random mutagenesis Site-directed mutagenesis Mutation induction Chimera dissociation Phenotypic screening Genotypic screening

Editors and affiliations

  • Joanna Jankowicz-Cieslak
    • 1
  • Thomas H. Tai
    • 2
  • Jochen Kumlehn
    • 3
  • Bradley J. Till
    • 4
  1. 1.Plant Breeding and Genetics LaboratoryJoint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria
  2. 2.Crops Pathology and Genetics Research UnitUSDA-ARSDavisUSA
  3. 3.Plant Reproductive BiologyLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany
  4. 4.Plant Breeding and Genetics LaboratoryJoint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria

Bibliographic information