In Vitro Fertilization

A Textbook of Current and Emerging Methods and Devices

  • Zsolt Peter Nagy
  • Alex C. Varghese
  • Ashok Agarwal

Table of contents

  1. Front Matter
    Pages I-XXII
  2. Yuval Or, Shir Dar, Zeev Shoham
    Pages 1-6
  3. Setting Up an ART Unit: Planning, Design and Organization

    1. Front Matter
      Pages 7-7
    2. Jacques Cohen, Mina Alikani, Antonia Gilligan, Tim Schimmel
      Pages 9-19
    3. Dean E. Morbeck, Marlena Duke
      Pages 21-27
    4. Johan Guns, Ronny Janssens
      Pages 29-37
    5. Julius Hreinsson, Kia Borg
      Pages 47-55
    6. Cecilia Sjoblom, Georgios Liperis
      Pages 57-68
  4. IVF Laboratory Equipment and Culture Systems

    1. Front Matter
      Pages 77-77
    2. Leslie Weikert, Christa Mackenzie, Klaus E. Wiemer
      Pages 79-84
    3. Marius Meintjes
      Pages 85-94
  5. Oocyte Preparation for IVF/ICSI

    1. Front Matter
      Pages 121-121
    2. Basak Balaban
      Pages 123-132
    3. Laura Francesca Rienzi, Roberta Maggiulli, Filippo Maria Ubaldi
      Pages 133-145
  6. Embryo Culture Methods

    1. Front Matter
      Pages 147-147
    2. David K. Gardner, Michelle Lane
      Pages 157-170
    3. Zhi-Yong Yang, Ling Wang, Ri-Cheng Chian
      Pages 171-182
    4. Vladimir Isachenko, Karl Sterzik, Gohar Rahimi, Peter Mallmann, Evgenia Isachenko
      Pages 207-212
    5. Peng Yuan, Liying Yan, Gary D. Smith
      Pages 213-225
    6. Andrey V. Dolinko, Catherine Racowsky
      Pages 227-245
  7. Sperm Assessment, Processing and Selection

    1. Front Matter
      Pages 247-247
    2. Tania R. Dias, Chak-Lam Cho, Ashok Agarwal
      Pages 249-263
    3. Tania R. Dias, Chak-Lam Cho, Ashok Agarwal
      Pages 265-281
    4. Ana D. Martins, Rakesh Sharma, Ashok Agarwal
      Pages 283-298
    5. Rocio Rivera-Egea, Nicolás Garrido, Alex C. Varghese
      Pages 299-312
    6. Sidney Verza Jr., Sandro C. Esteves
      Pages 313-334
    7. Rocio Rivera-Egea, Thamara Viloria Samochin, Marcos Meseguer Escrivá
      Pages 335-345
    8. Steven Fleming, Robert John Aitken
      Pages 347-352
    9. Enver Kerem Dirican
      Pages 353-358
  8. Insemination Procedures

    1. Front Matter
      Pages 359-359
    2. Gautam N. Allahbadia, Rubina Merchant, Akanksha Allahbadia, Goral Gandhi, Swati Allahbadia
      Pages 361-382
    3. Michael L. Reed
      Pages 383-397
    4. Gianpiero D. Palermo, Nigel Pereira, Queenie V. Neri, Stephen Chow, Stephanie Cheung, Zev Rosenwaks
      Pages 399-413
    5. Pierre Vanderzwalmen, Romain Imbert, David Jareno Martinez, Astrid Stecher, Anne Vansteenbrugge, Sabine Vanderzwalmen et al.
      Pages 415-428
    6. Marc Yeste, Celine Jones, Siti Nornadhirah Amdani, Kevin Coward
      Pages 429-445
  9. Micromanipulators and Micromanipulation

    1. Front Matter
      Pages 447-447
    2. Hubert Joris
      Pages 449-454
    3. Steven Fleming, Catherine Pretty
      Pages 455-463
    4. Kenichiro Hiraoka, Kiyotaka Kawai, Tatsuya Harada, Tomonori Ishikawa
      Pages 481-489
  10. Embryo Evaluation, Grading, and Assisted Hatching

    1. Front Matter
      Pages 491-491
    2. Lucía Alegre Ferri, Carmela Albert Rodríguez, Sonia Pérez Albalá, Marcos Meseguer Escrivá
      Pages 505-519

About this book


Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world.

With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.


ART unit Assisted hatching Assisted reproductive technology (ART) Blastocyst Cryopreservation Egg banking Embryo Embryo transfer Fertility preservation Gamete Infertility Intracytoplasmic sperm injection (ICSI) In vitro fertilization (IVF) In vitro maturation (IVM) Microfluidics Micromanipulation Oocyte Sperm Sperm selection Vitrification

Editors and affiliations

  • Zsolt Peter Nagy
    • 1
  • Alex C. Varghese
    • 2
  • Ashok Agarwal
    • 3
  1. 1.Reproductive Biology Associates – PreludeAtlantaUSA
  2. 2.Astra Fertility GroupMississaugaCanada
  3. 3.Cleveland ClinicClevelandUSA

Bibliographic information