Table of contents
About this book
This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, techniques involving RCA for enhanced signal amplification, novel RCA diagnostics, sensors for expediting RCA detection, and prospective RCA-based therapeutics. This is a valuable book for university professors and students in the field of biomedical engineering and biomolecular pharmacology as well as R&D managers of biotechnology and biopharmaceutical companies.
Specifically, this book:
- Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells
- Expands readers’ understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads
- Maximizes reader insights into novel RCA diagnostics, such as PNA openers-assisted RCA for detection of single target cells and in situ RCA diagnosis of cancer cells and malignant tissues
- Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification
Advance Praise for Rolling Circle Amplification (RCA):
“This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs.”
— Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA)
“In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts for building superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology.”
— Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas.
Coupled rolling circle amplification Enzymes Useful in RCA Improved variants of φ29 polymerase Intracellular Delivery of Immunostimulatory Drugs Novel RCA diagnostics PNA Openers-Assisted RCA Prospective RCA-based therapeutics RCA RCA-Based DNA Origami Nanostructrures RCA-LAMP RCA-Templated DNA Nanotubes RCA-based analysis of hepatitis B virus (HBV) RCA-directed enzyme-catalyzed polymerization Rolling circle amplification Sensors and detector probes for RCA detection Thermus thermophilus SSB mutant protein assays in nucleic acid diagnostics probe-tagged magnetic nanobeads prospective RCA-based therapeutics real-time monitoring of rolling-circle DNA amplification