Modern Proteomics – Sample Preparation, Analysis and Practical Applications

  • Hamid Mirzaei
  • Martin Carrasco

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 919)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Sample Preparation Strategies for Proteomics

    1. Front Matter
      Pages 1-1
    2. Anna Kwasnik, Claire Tonry, Angela Mc Ardle, Aisha Qasim Butt, Rosanna Inzitari, Stephen R. Pennington
      Pages 3-21
  3. Mass Spectrometry for Proteomics Analysis

    1. Front Matter
      Pages 145-145
    2. Kenneth Verheggen, Lennart Martens, Frode S. Berven, Harald Barsnes, Marc Vaudel
      Pages 147-156
    3. Anthony M. Haag
      Pages 157-169
  4. Bioinformatic Tools for Proteomics data Analysis and Interpretation

    1. Front Matter
      Pages 201-201
    2. Marius Cosmin Codrea, Sven Nahnsen
      Pages 203-215
    3. Thilo Muth, Erdmann Rapp, Frode S. Berven, Harald Barsnes, Marc Vaudel
      Pages 217-226
    4. Ragnhild R. Lereim, Eystein Oveland, Frode S. Berven, Marc Vaudel, Harald Barsnes
      Pages 227-235
    5. Zengyou He, Ting Huang, Can Zhao, Ben Teng
      Pages 237-242
    6. Robert J. Chalkley
      Pages 243-247
    7. Andre Bui, Maria D. Person
      Pages 249-253
    8. Yun Chen, Fuqiang Wang, Feifei Xu, Ting Yang
      Pages 255-279
    9. Karla Grisel Calderón-González, Jesús Hernández-Monge, María Esther Herrera-Aguirre, Juan Pedro Luna-Arias
      Pages 281-341
  5. Applications of Proteomics Technologies in Biological and Medical Sciences

    1. Front Matter
      Pages 343-343
    2. Benedetta Turriziani, Alexander von Kriegsheim, Stephen R. Pennington
      Pages 383-396
    3. Antonio Artigues, Owen W. Nadeau, Mary Ashley Rimmer, Maria T. Villar, Xiuxia Du, Aron W. Fenton et al.
      Pages 397-431
  6. Clinical Proteomics

    1. Front Matter
      Pages 433-433
    2. John E. Wiktorowicz, Allan R. Brasier
      Pages 435-441
    3. John E. Wiktorowicz, Kizhake V. Soman
      Pages 443-462
    4. Heidi M. Spratt, Hyunsu Ju
      Pages 463-492
    5. Yingxin Zhao, Allan R. Brasier
      Pages 493-514
    6. Andrew J. Percy, Juncong Yang, Andrew G. Chambers, Yassene Mohammed, Tasso Miliotis, Christoph H. Borchers
      Pages 515-530
  7. Back Matter
    Pages 531-533

About this book


This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of:

1. The types of samples can be analyzed by mass spectrometry for proteomics analysis.

2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis.

3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins.

4. How various mass spectrometers work and different ways they can be used for proteomics analysis

5. The various platforms that are available for proteomics data analysis

6. The various applications of proteomics technologies in biological and medical sciences

This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.


Protein Fractionation Protein Chromatography Plant Proteomics Bioinformatics Biomarkers

Editors and affiliations

  • Hamid Mirzaei
    • 1
  • Martin Carrasco
    • 2
  1. 1.UT Southwestern Medical CenterDallasUSA
  2. 2.Biotech DivisionNeurophagy Therapeutics, INCOdessaUSA

Bibliographic information