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Pancreatic Islet Isolation

From the Mouse to the Clinic

  • Miriam Ramírez-Domínguez

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 938)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Miriam Ramírez-Domínguez
    Pages 1-9
  3. Midhat H. Abdulreda, Rayner Rodriguez-Diaz, Over Cabrera, Alejandro Caicedo, Per-Olof Berggren
    Pages 11-24
  4. Miriam Ramírez-Domínguez
    Pages 25-34
  5. Heide Brandhorst, Paul R. V. Johnson, Daniel Brandhorst
    Pages 35-55
  6. Wayne J. Hawthorne
    Pages 67-88
  7. Wayne J. Hawthorne, Lindy Williams, Yi Vee Chew
    Pages 89-122
  8. Back Matter
    Pages 123-123

About this book

Introduction

Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate “work of a craftsman”. This book, which is aimed at beginners and experts alike, is a survey of the current state-of–the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevant species (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.

Keywords

Animal Models Diabetes Human Islet Isolation Islet Purification Translational Studies

Editors and affiliations

  • Miriam Ramírez-Domínguez
    • 1
  1. 1.Laboratory of Cell Therapy of Diabetes, Department of Pediatrics, Faculty of Medicine and Odontology, Hospital CrucesUniversity of the Basque Country (UPV/EHU)LeioaSpain

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-319-39824-2
  • Copyright Information Springer International Publishing Switzerland 2016
  • Publisher Name Springer, Cham
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-3-319-39822-8
  • Online ISBN 978-3-319-39824-2
  • Series Print ISSN 0065-2598
  • Series Online ISSN 2214-8019
  • Buy this book on publisher's site