Advertisement

Table of contents

  1. Front Matter
    Pages i-x
  2. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 1-4 Open Access
  3. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 5-7 Open Access
  4. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 9-11 Open Access
  5. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 13-17 Open Access
  6. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 19-20 Open Access
  7. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 21-22 Open Access
  8. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 23-26 Open Access
  9. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 27-33 Open Access
  10. Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
    Pages 35-35 Open Access

About this book

Introduction

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Keywords

Crop improvement DNA extraction Induced mutations Low-cost assays Mutant germplasm characterization Nucleotide variation in plant genomes PCR amplification

Authors and affiliations

  • Bradley J. Till
    • 1
  • Joanna Jankowicz-Cieslak
    • 2
  • Owen A. Huynh
    • 3
  • Mayada M. Beshir
    • 4
  • Robert G. Laport
    • 5
  • Bernhard J. Hofinger
    • 6
  1. 1.Plant Breeding and Genetics LaboratoryJoint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria
  2. 2.Plant Breeding and Genetics LaboratoryJoint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria
  3. 3.Plant Breeding and Genetics LaboratoryJoint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria
  4. 4.Agricultural Research CorporationKhartoum NorthSudan
  5. 5.School of Biological SciencesUniversity of Nebraska-LincolnLincolnUSA
  6. 6.Plant Breeding and Genetics LaboratoryJoint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureViennaAustria

Bibliographic information