© 2014

Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo


Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Thomas Harry Sharp
      Pages 3-56
  3. Materials and Methods

    1. Front Matter
      Pages 57-57
    2. Thomas Harry Sharp
      Pages 59-72
    3. Thomas Harry Sharp
      Pages 73-82
  4. Experimental Results

    1. Front Matter
      Pages 83-83
  5. Back Matter
    Pages 145-150

About this book


As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his PhD, Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.


Coiled Coils Correlative Light Electron Microscopy Cryo Electron Microscopy Electron Crystallography Fibrous Proteins Helical Reconstruction Metallothionein Protein Design Single-particle Analysis

Authors and affiliations

  1. 1.School of ChemistryUniversity of BristolBristolUnited Kingdom

About the authors

Thom Sharp completed his bachelor’s degree in Biochemistry at the University of Bristol, with a year spent a year working at AstraZeneca. He continued in Bristol for his PhD in bionanotechnology, which was performed under the supervision of Dek Woolfson, a protein design chemist, and Paul Verkade, an electron microscopist. Thom is currently at the University of Oxford, where he is developing DNA-templated protein arrays to increase the throughput of single-particle analysis.

Bibliographic information

  • Book Title Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo
  • Authors Thomas Harry Sharp
  • Series Title Springer Theses
  • Series Abbreviated Title Springer Theses
  • DOI
  • Copyright Information Springer International Publishing Switzerland 2014
  • Publisher Name Springer, Cham
  • eBook Packages Chemistry and Materials Science Chemistry and Material Science (R0)
  • Hardcover ISBN 978-3-319-02158-4
  • Softcover ISBN 978-3-319-35023-3
  • eBook ISBN 978-3-319-02159-1
  • Series ISSN 2190-5053
  • Series E-ISSN 2190-5061
  • Edition Number 1
  • Number of Pages XVIII, 150
  • Number of Illustrations 30 b/w illustrations, 55 illustrations in colour
  • Topics Analytical Chemistry
    Biological Microscopy
  • Buy this book on publisher's site