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Catalytically Active Nucleic Acids

  • Harald Seitz
  • Frank Stahl
  • Johanna-Gabriela Walter
Book

Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 170)

Table of contents

  1. Front Matter
    Pages i-vii
  2. Samuel Rouleau, Rachel Jodoin, Jean-Michel Garant, Jean-Pierre Perreault
    Pages 1-20
  3. Darko Balke, Robert Hieronymus, Sabine Müller
    Pages 21-35
  4. Fatemeh Javadi-Zarnaghi, Claudia Höbartner
    Pages 37-58
  5. Back Matter
    Pages 121-123

About this book

Introduction

This volume reviews numerous reaction mechanisms and applications of nucleic acids with catalytic activity. Written by an interdisciplinary team of authors, it provides an essential overview of these acids’ fundamental aspects, while also addressing associated methodologies such as nucleic acid enzyme engineering, peroxidase-mimicking DNAzymes and Aptazymes.

After the discovery of natural ribozymes – RNA molecules that mediate the cleavage and formation of phosphodiester bonds and the formation of peptide bonds – numerous artificial ribozymes with altered catalytic activities were produced by in vitro and in vivo selection. Unlike ribozymes, DNAzymes do not occur in nature. Although the catalytic activity of nucleic acid enzymes is usually much slower than that of proteins, nucleic acid enzymes with comparable catalytic activity have been obtained using stringent selection processes. The key advantages of these enzymes: they are e.g. smaller, easier to produce and purify than proteins, and can withstand denaturation, e.g. by heat.

Over the last few years, the number of publications on the applications of enzymatic nucleic acids has grown steadily. Summarizing the fundamentals and applications of these acids, this book will not only be an excellent resource for experts in the field but will also guide young researchers just starting out in this significant area.

Keywords

RNA G-Quadruplexes DNAzymes nucleic acids Aptazymes Transcriptome RNaseP Ribozyme DNAzyme Engineering RNA Aptamers

Editors and affiliations

  • Harald Seitz
    • 1
  • Frank Stahl
    • 2
  • Johanna-Gabriela Walter
    • 3
  1. 1.Branch Bioanalytics and Bioprocesses (IZI-BB) Biomarker Validation and Assay DevelopmentFraunhofer Institute for Cell Therapy and ImmunologyPotsdamGermany
  2. 2.Institut für Technische ChemieLeibniz Universität HannoverHannoverGermany
  3. 3.Institut für Technische ChemieLeibniz Universität HannoverHannoverGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-3-030-29646-9
  • Copyright Information Springer Nature Switzerland AG 2020
  • Publisher Name Springer, Cham
  • eBook Packages Chemistry and Materials Science
  • Print ISBN 978-3-030-29645-2
  • Online ISBN 978-3-030-29646-9
  • Series Print ISSN 0724-6145
  • Series Online ISSN 1616-8542
  • Buy this book on publisher's site