In Vitro Neuronal Networks

From Culturing Methods to Neuro-Technological Applications

  • Michela Chiappalone
  • Valentina Pasquale
  • Monica Frega

Part of the Advances in Neurobiology book series (NEUROBIOL, volume 22)

Table of contents

  1. Front Matter
    Pages i-xv
  2. In Vitro Neuronal Cultures: Experimental Models and Nanomaterials

    1. Front Matter
      Pages 1-1
    2. Jason M. Keller, Monica Frega
      Pages 3-17
    3. Joost le Feber
      Pages 19-49
    4. Mattia Bramini, Anna Rocchi, Fabio Benfenati, Fabrizia Cesca
      Pages 51-79
  3. Recording Techniques

    1. Front Matter
      Pages 81-81
    2. Micha E. Spira, Shun-Ho Huang, Nava Shmoel, Hadas Erez
      Pages 125-153
    3. Andrea Spanu, Mariateresa Tedesco, Sergio Martinoia, Annalisa Bonfiglio
      Pages 155-167
  4. Data Analysis Methods

    1. Front Matter
      Pages 169-169
    2. Matthias H. Hennig, Cole Hurwitz, Martino Sorbaro
      Pages 171-184
    3. Ellese Cotterill, Stephen J. Eglen
      Pages 185-206
  5. Applications

    1. Front Matter
      Pages 251-251
    2. Davide Lonardoni, Hayder Amin, Stefano Zordan, Fabio Boi, Aziliz Lecomte, Gian Nicola Angotzi et al.
      Pages 253-273
    3. Laura Ylä-Outinen, Jarno M. A. Tanskanen, Fikret E. Kapucu, Anu Hyysalo, Jari A. K. Hyttinen, Susanna Narkilahti
      Pages 299-329
    4. Marta Bisio, Alexey Pimashkin, Stefano Buccelli, Jacopo Tessadori, Marianna Semprini, Timothée Levi et al.
      Pages 351-387

About this book


This book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies.


stem cell cultures coating patterning Micro-Electrode Array neural interfaces multi-channel acquisition and processing primary cell cultures

Editors and affiliations

  • Michela Chiappalone
    • 1
  • Valentina Pasquale
    • 2
  • Monica Frega
    • 3
  1. 1.Rehab Technologies IIT-INAIL Joint LabIstituto Italiano di TecnologiaGenovaItaly
  2. 2.Neuroscience and Brain TechnologiesIstituto Italiano di TecnologiaGenovaItaly
  3. 3.Department of Clinical NeurophysiologyUniversity of TwenteEnschedeThe Netherlands

Bibliographic information